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Somatostatin acts through G-proteins on dopaminergic adenylate cyclase in the caudate-putamen of the rat

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Abstract

Somatostatin was incubated in an adenylate cyclase assay of a particulate fraction of caudateputamen tissue of the rat in order to examine the effect of the peptide on D-1 receptor coupled adenylate cyclase in vitro. Somatostatin was able to enhance cyclic AMP formation in the presence of guanylylimidodiphosphate and guanosine-triphosphate. In contrast to this, somatostatin inhibited both dopamine and forskolin-stimulated cyclic AMP accumulation. Pertussis toxin and cholera toxin also depressed forskolin-induced stimulation. Somatostatin was found to antagonize these inhibitory effects of pertussis toxin and cholera toxin. The results suggest that somatostatin acts through a stimulatory as well as an inhibitory guanine nucleotide regulatory protein subtype to affect dopaminergic adenylate cyclase activity.

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Moser, A., Cramer, H. Somatostatin acts through G-proteins on dopaminergic adenylate cyclase in the caudate-putamen of the rat. Neurochem Res 15, 1085–1087 (1990). https://doi.org/10.1007/BF01101708

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Key Words

  • Adenylate cyclase
  • somatostatin
  • G-proteins
  • caudate-putamen
  • rat