Somatostatin was incubated in an adenylate cyclase assay of a particulate fraction of caudateputamen tissue of the rat in order to examine the effect of the peptide on D-1 receptor coupled adenylate cyclase in vitro. Somatostatin was able to enhance cyclic AMP formation in the presence of guanylylimidodiphosphate and guanosine-triphosphate. In contrast to this, somatostatin inhibited both dopamine and forskolin-stimulated cyclic AMP accumulation. Pertussis toxin and cholera toxin also depressed forskolin-induced stimulation. Somatostatin was found to antagonize these inhibitory effects of pertussis toxin and cholera toxin. The results suggest that somatostatin acts through a stimulatory as well as an inhibitory guanine nucleotide regulatory protein subtype to affect dopaminergic adenylate cyclase activity.
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Eckernäs, S. A., Aquilonius, S. M., Lundquist, G., and Roose, B. E. 1978. Regional distribution of cholinergic and monoaminergic markers, somatostatin and substance P in the human brain. Acta Neurol Scand 57:225–226.
Hökfelt, T., Johanson, C., Ljungdahl, J. M., and Schultzberg, M. 1980. Peptidergic neurons. Nature 284:515–521.
Rodbell, M., Lad, P. M., Nielson, T. B., Cooper, D. M. F., Schlegel, W., Preston, M. S., Londos, C., and Kempner, E. S. 1981. The structure of adenylate cyclase systems. Adv Cyclic Nucl Res 14:3–14.
Jakobs, K. H., and Schultz, G. 1983. Occurrence of a hormone-sensitive inhibitory coupling component of the adenylate cyclase in S 49 lymphoma cyc variants. Proc Natl Acad Sci 80:3899–3902.
Moser, A., Reavill, C., Jenner, P., Marsden, C. D., and Cramer, H. 1986. Effects of somatostatin on dopamine sensitive adenylate cyclase activity in the caudate-putamen of the rat. Exp Brain Res 62:567–571.
Moser, A., Reavill, C., Liebetrau, A., Jenner, P., Marsden, C. D., and Cramer, H. 1987. Dopamine and somatostatin modulated adenylate cyclase activity in the rat caudate-putamen following unilateral cortical ablation. Exp Brain Res 68:406–410.
Bensadoun, A., and Weinstein, D. 1976. Assay of protein in the presence of interfering materials. Anal Biochem 70:241–250.
Birnbaumer, L., Swartz, T. L., Abramowitz, J., Mintz, P. W., and Iyengar, R. 1980. Transient and stady state kinetics or the interaction of guanyl nucleotides with the adenylate cyclase system from rat liver plasma membranes. J. Biol Chem 255:3542–3551.
Salomon, Y. 1979. Adenylate cyclase assay. Adv Cyclic Nucl Res 10:35–55.
Cohn, M. L. and Cohn, M. 1975. “Barrel rotation” induced by somatostatin in the non-lesioned rat. Brain Res 96:138–141.
Garcia-Sevilla, J. A., Magnusson, T., and Carlsson, A. 1978. Effect of intracerebroventricularly administered somatostatin on brain monamine turnover. Brain Res 155:159–164.
Reisine, T. D., Zhan, Y., and Sekura, R. D. 1983. Pertussis toxin blocks somatostatin's inhibition of stimulated cyclic AMP accumulation in anterior pituitary tumor cells. Biochem Biophys Res Commun 115:794–799.
Seamon, B., Padgett, W., and Daly, J. W. 1981. Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells. Proc Natl Acad Sci 78:3363–3367.
Markstein, R., Stöckli, K. A., and Reubi, J. C. 1989. Differential effects of somatostatin on adenylate cyclase as functional correlate for different brain somatostatin receptor subpopulations. Neuroscience Letters 104:13–18.
Hildebrandt, J. D., Sekura, R. D., Codina, J., Iyengar, R., Manclark, C. R., and Birnbaumer, L. 1983. Stimulation and inhibition of adenylyl cyclases mediated by distinct regulatory proteins. Nature 302:707–709.
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Moser, A., Cramer, H. Somatostatin acts through G-proteins on dopaminergic adenylate cyclase in the caudate-putamen of the rat. Neurochem Res 15, 1085–1087 (1990). https://doi.org/10.1007/BF01101708
- Adenylate cyclase