It was recently shown that the site of photoinhibition in leaves ofCucumis sativus L. at low temperatures is Photosystem I (PSI), not PSII (I. Terashima et al. 1994, Planta193, 300–306). In the present study, the mechanisms of this PSI photoinhibition in vivo were examined. By lowering the photon flux density during the photoinhibitory treatment of leaves at 4°C for 5 h to less than 100 μmol·m−2s−1, we were able to separate the steps of the destruction of the electron-transfer components. Although P-700, the reaction-center chlorophyll, was almost intact in this low-light treatment, the quantum yield of the electron transfer through PSI and photochemically induced absorption change at 701 nm were markedly inhibited. This, along with the results from the measurements of the light-induced absorption changes in the presence of various concentrations of methyl viologen, an artificial electron acceptor, indicates that the component on the acceptor side of the PSI, A1 or Fx, is the first site of inactivation. When the photon flux density during the treatment was increased to 220 μmol·m−2s−1, the destruction of P-700 itself was also observed. Furthermore, the partial degradation of the chlorophyll-binding large subunits was observed in photoinhibited leaves. This degradation of the subunits was not detected when the treatment was carried out under nitrogen atmosphere, the condition in which the electron transfer is not inhibited. Thus, the photoinhibitory processes in the reaction center of PSI go through three steps, the inactivation of the acceptor side, the destruction of the reaction-center chlorophyll and the degradation of the reaction center subunit(s). The similarities and the differences between the mechanisms of PSI photoinhibition and those of PSII photoinhibition are discussed.
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- LHCI, LHCII:
light-harvesting chlorophyll-a/b proteins associating with photosystems I and II, respectively
photon flux density
Aro, E.-M., Hundal, T., Carlberg, I., Andersson, B. (1990) In vitro studies on light-induced inhibition of Photosystem II and D1-protein degradation at low temperatures. Biochim. Biophys. Acta1019, 269–275
Aro, E.-M., Virgin, I., Andersson, B. (1993) Photoinhibition of Photosystem II. Inactivation, protein damage and turnover. Biochim. Biophys. Acta1143, 113–134
Asada, K., Takahashi, M. (1987) Production and scavenging of active oxygen in photosynthesis. In: Photoinhibition. pp. 227–287, Kyle, D.J., Osmond, C.B., Arntzen, C.J. eds., Elsevier, Amsterdam
Davis, B.J. (1964) Disc electrophoresis — II Method and application to human serum proteins. Annu. N.Y. Acad. Sci.121, 404–427
Ellis, R.J. (1981) Chloroplast proteins: Synthesis, transport, and assembly. Annu. Rev. Plant Physiol.32, 111–137
Golbeck, J.H., Bryant, D. (1991) Photosystem I. Curr. Top. Bioenerg.16, 83–177
Havaux, M., Davaud, A. (1994) Photoinhibition of photosynthesis in chilled potato leaves is not correlated with a loss of photosystem-II activity: Preferential inactivation of photosystem I. Photosynth. Res., in press
Hodgson, R.A.J., Raison, J.K. (1989) Inhibition of photosynthesis by chilling in moderate light: a comparison of plants sensitive and insensitive to chilling. Planta178, 545–552
Hodgson, R.A.J., Orr, G.R., Raison, J.K. (1987) Inhibition of photosynthesis by chilling in the light. Plant Sci.49, 75–79
Ikeuchi, M., Inoue, Y. (1988) A new 4.8-kDa polypeptide intrinsic to the PS II reaction center as revealed by modified SDS-PAGE with improved resolution of low-molecular-weight proteins. Plant Cell Physiol.29, 1233–1239
Inoue, K., Sakurai, H., Hiyama, T. (1986) Photoinactivation sites of photosystem I in isolated chloroplasts. Plant Cell Physiol.27, 961–968
Inoue, K., Fujii, T., Yokoyama, E., Matsuura, K., Hiyama, T., Sakurai, H. (1989) The photoinhibition site of Photosystem I in isolated chloroplasts under extremely reducing conditions. Plant Cell Physiol.30, 65–71
Kashino, Y., Enami, I., Satoh, K., Katoh, S. (1990) Immunological cross-reactivity among corresponding proteins of Photosystem I and II from widely divergent photosynthetic organisms. Plant Cell Physiol.31, 479–488
Melis, A. (1991) Dynamics of photosynthetic membrane composition and function. Biochim. Biophys. Acta1058, 87–106
Porra, R.J., Thompson, W.A., Kriedemann, P.E. (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophyllsa andb extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim. Biophys. Acta975, 384–394
Powles, S.B. (1984) Photoinhibition of photosynthesis induced by visible light. Annu. Rev. Plant Physiol.35, 15–44
Sassenrath, G.F., Ort, D.R., Portis, A.R., Jr. (1990) Impaired reductive activation of stromal bisphosphatases in tomato leaves following low-temperature exposure at high light. Arch. Biochem. Biophys.282, 302–308
Satoh, Ki. (1970a) Mechanism of photoinactivation in photosynthetic systems. I. The dark reaction in photoinactivation. Plant Cell Physiol.11, 15–27
Satoh, Ki. (1970b) Mechanism of photoinactivation in photosynthetic systems. II. The occurrence and properties of two different types of photoinactivation. Plant Cell Physiol.11, 29–38
Satoh, Ki. (1970c) Mechanism of photoinactivation in photosynthetic systems. III. Site and mode of photoinactivation in photosystem I. Plant Cell Physiol.11, 187–197
Satoh, Ka., Fork, D.C. (1982) Photoinhibition of reaction centers of photosystems I and II in intactBryopsis chloroplasts under anaerobic conditions. Plant Physiol.70, 1004–1008
Setif, P., Bottin, H. (1989) Identification of electron-transfer reactions involving the acceptor A1 of Photosystem I at room temperature. Biochemistry28, 2689–2697
Sonoike, K., Katoh, S. (1988) Effects of sodium dodecyl sulfate and methyl viologen on the chlorophylla associated with oxidation of P-700. Biochim. Biophys. Acta935, 61–71
Takahashi, Y., Katoh, S. (1984) Triplet states in a Photosystem I reaction center complex. Inhibition of radical pair recombination by bipyridinium dyes and naphthoquinones. Plant Cell Physiol.25, 785–794
Terashima, I., Kashino, Y., Katoh, S. (1991) Exposure of leaves ofCucumis sativus L. to low temperatures in the light causes uncoupling of thylakoids. I. Studies with isolated thylakoids. Plant Cell Physiol.32, 1267–1274
Terashima, I., Funayama, S., Sonoike, K. (1994) The site of photoinhibition in leaves ofCucumis sativus L. at low temperatures is photosystem I, not photosystem II. Planta193, 300–306
van Mieghem, F.J.E., Nitschke, W., Mathis, P., Rutherford, A.W. (1989) The influence of the quinone-iron electron acceptor complex on the reaction centre photochemistry of photosystem II. Biochim. Biophys. Acta977, 207–214
We are grateful to Dr. I. Enami (Department of Biology, Faculty of Science, Science University of Tokyo) and Drs. H. Matsubara and H. Oh-oka (Department of Biology, Faculty of Science, Osaka University) for generous gifts of antisera used in the present work. We also thank A. Aoyama for technical assistance. This work was partly supported by the grants from the Ministry of Education, Science and Culture, Japan.
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Sonoike, K., Terashima, I. Mechanism of photosystem-I photoinhibition in leaves ofCucumis sativus L.. Planta 194, 287–293 (1994). https://doi.org/10.1007/BF01101690
- Chilling stress
- Cucumis (photoinhibition)
- Photosystem I
- Subunit protein degradation