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Mechanism of photosystem-I photoinhibition in leaves ofCucumis sativus L.

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Abstract

It was recently shown that the site of photoinhibition in leaves ofCucumis sativus L. at low temperatures is Photosystem I (PSI), not PSII (I. Terashima et al. 1994, Planta193, 300–306). In the present study, the mechanisms of this PSI photoinhibition in vivo were examined. By lowering the photon flux density during the photoinhibitory treatment of leaves at 4°C for 5 h to less than 100 μmol·m−2s−1, we were able to separate the steps of the destruction of the electron-transfer components. Although P-700, the reaction-center chlorophyll, was almost intact in this low-light treatment, the quantum yield of the electron transfer through PSI and photochemically induced absorption change at 701 nm were markedly inhibited. This, along with the results from the measurements of the light-induced absorption changes in the presence of various concentrations of methyl viologen, an artificial electron acceptor, indicates that the component on the acceptor side of the PSI, A1 or Fx, is the first site of inactivation. When the photon flux density during the treatment was increased to 220 μmol·m−2s−1, the destruction of P-700 itself was also observed. Furthermore, the partial degradation of the chlorophyll-binding large subunits was observed in photoinhibited leaves. This degradation of the subunits was not detected when the treatment was carried out under nitrogen atmosphere, the condition in which the electron transfer is not inhibited. Thus, the photoinhibitory processes in the reaction center of PSI go through three steps, the inactivation of the acceptor side, the destruction of the reaction-center chlorophyll and the degradation of the reaction center subunit(s). The similarities and the differences between the mechanisms of PSI photoinhibition and those of PSII photoinhibition are discussed.

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Abbreviations

DAD:

2,3,5,6-tetramethyl-p-phenylenediamine

LHCI, LHCII:

light-harvesting chlorophyll-a/b proteins associating with photosystems I and II, respectively

PFD:

photon flux density

References

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Additional information

We are grateful to Dr. I. Enami (Department of Biology, Faculty of Science, Science University of Tokyo) and Drs. H. Matsubara and H. Oh-oka (Department of Biology, Faculty of Science, Osaka University) for generous gifts of antisera used in the present work. We also thank A. Aoyama for technical assistance. This work was partly supported by the grants from the Ministry of Education, Science and Culture, Japan.

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Sonoike, K., Terashima, I. Mechanism of photosystem-I photoinhibition in leaves ofCucumis sativus L.. Planta 194, 287–293 (1994). https://doi.org/10.1007/BF01101690

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Key words

  • Chilling stress
  • Cucumis (photoinhibition)
  • Photoinhibition
  • Photosynthesis
  • Photosystem I
  • Subunit protein degradation