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The effect of algal protein diets on the regeneration of serum and liver proteins in protein depleted rats

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Abstract

The protein quality of algaeSpirulina platensis was evaluated biochemically in adult albino rats by serum protein regeneration studies. Rats depleted for 12 days were repleted for 15 days with diets containing 10% protein derived from algae, methionine supplemented algae, or casein. On protein depletion, the activity of the hepatic enzymes, succinic dehydrogenase and alanine aminotransferase, were lowered while the activity of alkaline phosphatase increased. Protein repletion with algal and casein diets resulted in an increased activity of succinic dehydrogenase and alanine amino transferase and a decrease in alkaline phosphatase activity. However, the levels of these three enzymes were lower with the algal than with the casein diets. Algal diets regenerated serum protein effectively though to a slightly lesser extent than casein.

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References

  1. 1.

    Anusuya M (1982) Biochemical and Nutritional Studies on Algal Proteins. PhD Thesis. University of Mysore, India, 234 pp.

  2. 2.

    Becker EW, Venkataraman LV (1982) The Biotechnology and Exploitation of Algae. The Indian Approach. GTZ D-6236 Eschborn, Federal Republic of Germany, pp 17–76

  3. 3.

    Bourges H, Sotomayor A, Mendoza E Chavez (1971) Utilization of the algaSpirulina as a protein source. Nut Rep Int 4:31–43

  4. 4.

    Brin M, Mekee RM (1956) Effects of X-irradiation nitrogen mustard fenting cartisone and adrenalectomy on transaminase activity in the rats. Arch Biochem Biophy 61:384–389

  5. 5.

    Caldwell EF, Metterny EW (1953) Studies on vitamin B6 and transamination in rat liver. Arch Biochem Biophys 45:97–104

  6. 6.

    Chapman DC, Castillo R, Campbell JA (1959) A method for the determination of protein efficiency ratios. Can J Biochem Physiol 37:679–686

  7. 7.

    Clement G, Giddey C, Menzi R (1967) Amino acid composition and nutritive value of the algaSpirulina maxima. J Sci Fd Agric 18:497–501

  8. 8.

    Dam R, Lee L, Fry PC, Fox H (1965) Utilization of algae as a protein source of human. J Nut 86:376–382

  9. 9.

    Davis AJ (1964) Deic electrophoresis II. Method and application to human serum proteins. Ann NY Acad Sci 121:404–427

  10. 10.

    Flodin NW (1953) Amino acids and proteins. Their place in human nutrition problems. J Agric Fd Chem 1:222–229

  11. 11.

    Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the Biuret Reaction. J Biol Chem 177:751–766

  12. 12.

    Guttikar MN, Panemangalore M, Narayana Rao M (1970) Effects of protein calorie ratio on liver enzyme concentration in young weanling rats. Nut and Metabolism 12:136–143

  13. 13.

    Heard CRC, Frangi SM, Wright PM, McCartney PR (1977) Biochemical characteristics of different forms of protein energy malnutrition an experimental model using rats. Brit J Nut 37:1–21

  14. 14.

    Hubbel RB, Mendel LB, Wakeman AJ (1937) A new salt mixture for use in experimental diets. J Nutr 14:273–285

  15. 15.

    Hubscher G, West GR (1965) Specific assays of some phosphatase in subcellular fractions of small intestine mucosa. Nature 205:799–800

  16. 16.

    Jaya TV, Venkataraman LV (1980) Serum protein regeneration studies on rats fed diets containing germinated legumes. Ind J Nut and Dietet 17:171–177

  17. 17.

    Kraut H, Rolle S (1968) Amounts of vitamin B1 and B2 present in the green algaScenedesmus obliqus. Nutr Dieta 10:183–193

  18. 18.

    Kun E, Aboode AJ (1949) Calorimetric estimation of succinic dehydrogenase by triphenyl tetrazolium chloride. Science 109:144–146

  19. 19.

    Lubitz JA (1963) The protein quality, digestibility and composition of algaeChlorella. J Fd Sci 28:229–232

  20. 20.

    Miller LL (1950) The loss and regeneration of rat liver enzymes related to diet proteins. J Biol Chem 186:253–260

  21. 21.

    Muramatsu K, Ashida K (1962) Effect of dietary protein level on growth and liver enzymes activities of rats. J Nut 76:143–150

  22. 22.

    Pabst W, Jekat J, Rolle I (1964) The utilization of the carbohydrates, fats, phosphorus and nitrogen from roller driedScenedesmus substance as shown by balance studies in the rat. Nut and Dieta 6:279–284

  23. 23.

    Pearson D (1970) “The Chemical Analysis of Food” 6th ed. Churchill, London

  24. 24.

    Powell RC, Nevels EM, McDowell ME (1961) Algae feeding in humans. J Nut 75:7–12

  25. 25.

    Richard D, Lee S, Fry PG, Fox H (1965) Utilization of algae as a protein source of human. J Nutr 86:376–382

  26. 26.

    Rosenthal O, Fahl JC, Vars HM (1952) Response of alkaline phosphatase of rat liver to protein depletion and inanition. J Biol Chem 194:299–309

  27. 27.

    Singh KN, Mittal RK, Barthwal KC, Agrawall GC (1976) Repletion studies on liver protein, glycogen content and liver enzymes by feeding protein isolates of Cassia Margirata and Cassia Renigera wild leguminous seeds in normal albino rats. Ind J Nut & Dietet 13:142–247

  28. 28.

    Srinivasan PR, Patwardhan VN (1955) The effect of deficiency on (a) some liver constituents and (b) enzymes in liver pancrease and blood plasma in albino rats. Ind J Med Res 43:1–13

  29. 29.

    Taussky HH, Shorr E (1953) A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem 202:675–685

  30. 30.

    Tohanzy NE, White NG, Umbriet WW (1950) A rapid method for the estimation of the glutamic aspartic transaminase in tissue and its application to radiation sickness. Arch Biochem Biophys 28:36–42

  31. 31.

    Venkataraman LV, Becker EW, Shamala TR (1977) Studies on the cultivation and utilization of the algaScenedesmus acutus as a single cell protein. Life Sci 20: 223–234

  32. 32.

    Venkataraman LV (1978) New possibilities of microalgae production and utilization in India. Arch Hydrobiol Beih Ergebn Linrol 11:199–210

  33. 33.

    Venkataraman LV, Nigam BP (1979) Mass culturing of fresh water algae for utilization as a source of protein. Phykos 18:83–95

  34. 34.

    Venkataraman LV (1983) Monograph on blue green algaSpirulina plantensis. Biotechnology and application. Central Food Technological Research Institute, Mysore, India, pp 1–33

  35. 35.

    Williams MA, Briggs GM (1963) An evaluation of mineral mixtures commonly used in diets for experimental animals. Amer J Clin Nutr 13:115–121

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Anusuya Devi, M., Venkataraman, L.V. The effect of algal protein diets on the regeneration of serum and liver proteins in protein depleted rats. Plant Food Hum Nutr 33, 287–297 (1983). https://doi.org/10.1007/BF01094754

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Key words

  • algae
  • serum protein regeneration
  • depletion
  • repletion
  • albino rats
  • liver enzymes and algal proteins