Plant Foods for Human Nutrition

, Volume 49, Issue 3, pp 241–252 | Cite as

Levels of antinutritional factors in pearl millet as affected by processing treatments and various types of fermentation

  • Alka Sharma
  • A. C. Kapoor
Article

Abstract

Pearl millet (Pennisetum typhoideum) was fermented with Lactobacilli or yeasts alone and in combination, and with natural microflora after various processing treatments, as grinding, soaking, debranning, dry heat treatment, autoclaving and germination. Fermentation was carried out at 30°C for 48 hours withLactobacillus plantarum (LP) andRhodotorula (R) isolated from naturally fermented pearl millet andLactobacillus acidophilus (LA),Candida utilis (CU) and natural microflora (NF). Germination and autoclaving, and debranning and autoclaving were the most effective processing treatments to reduce the phytic acid, amylase inhibitors and polyphenols. There was a further reduction in these antinutrients due to fermentation. Phytic acid and amylase inhibitors were completely eliminated after fermentation in some of the samples especially in soaked, debranned and germinated ones. Polyphenols were altered non-significantly in general but fermentation with Lp+R and NF caused a significant increased in polyphenols.

Key words

Pearl millet Processing Fermentation Yeast Lactobacilli Antinutrients 

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References

  1. 1.
    Rai KN, Anand Kumar K (1994) Pearl millet improvement at ICRISAT — An update. Feature article. International Sorghum and Millets Newsletter 35: 1–29.Google Scholar
  2. 2.
    Sutardi Buckle KA (1985) Reduction in phytic acid levels in soybeans during tempeh production, storage and frying. J Food Sci 50: 260–261.Google Scholar
  3. 3.
    Aggarwal AJ (1992) Processing of pearl millet for its more effective utilisation Ph.D. Thesis CCS Haryana Agricultural University, Hisar, India.Google Scholar
  4. 4.
    Thompson LU, Yoon JH (1984) Starch digestibility as affected by polyphenol and phytic acid. J Food Sci 49: 1228–1229.Google Scholar
  5. 5.
    Pawar VD, Parlikar GS (1990) Reducing the polyphenols and phytate and improving the protein quality of pearl millet by dehulling and soaking. J Food Sci Technol 27: 140–143.Google Scholar
  6. 6.
    Henry RJ, Battershell VG, Brennan PS, Oono K (1992) Control of wheat alpha-amylase by using inhibitors from cereals. J Sci Food Agric 58: 281–284.Google Scholar
  7. 7.
    Udupa SL, Prabhakar AR, Tandon S (1989) Alpha-amylase inhibitors in food stuffs. Food Chem 34: 95–101.Google Scholar
  8. 8.
    Shivraj B, Pattabiraman TN (1980) Natural plant enzyme inhibitors. Part VIII—Purification and properties of two alpha-amylase inhibitors from ragi (Eleusine coracana) grains. India J Biochem Biophys 17: 181–185.Google Scholar
  9. 9.
    Sharma KK, Pattabiraman TN (1982) Natural plant enzyme inhibitors. Purification and properties of an amylase-inhibitor from yam (Diascorea alata). J Sci Food Agric 33: 255–262.Google Scholar
  10. 10.
    Feng GH, Chen M, Kramer KJ, Reeck GR (1991) Alpha-amylase inhibitors from rice: Fractionation and selectivity toward insect, mammalian and bacterial alpha-amylase. Cereal Chem 68(5): 516–521.Google Scholar
  11. 11.
    Tabekhia MM, Luh BS (1980) Effect of germination, cooking and canning on phosphorus and phytate retention in dry beans. J Food Sci 45: 406–408.Google Scholar
  12. 12.
    Deshpande SS, Cheryan M (1983) in phytic acid, tannins and trypsin activity on soaking of dry beans (Phaseolus vulgaris) Nutr Rep Int 27: 371.Google Scholar
  13. 13.
    Duhan A, Chauhan BM, Punia D, Kapoor AC (1989) Phytic acid content of chickpea (Cicer arietinum) and black gram (Vigna mungo): Varietal differences and effect of domestic processing and cooking methods. J Sci Food Agric 49: 449–455.Google Scholar
  14. 14.
    Grewal RB (1992) Nutritional improvement of soyabean through fermentation and its utilisation in traditional foods of India. Ph.D. Thesis, CCS Haryana Agricultural University, Hisar, India.Google Scholar
  15. 15.
    Akinyele TO (1989) Effects of traditional methods of processing on the nutrient content and some antinutritional factors in cowpea (Vigna unguiculata). Food Chem 33: 291–299.Google Scholar
  16. 16.
    Kakker S (1992) Bioavailability of minerals in chickpea: Effect of domestic processing and level of the antinutrients. Ph.D. Thesis. CCS Haryana Agricultural University, Hisar, India.Google Scholar
  17. 17.
    Khetarpaul N, Chauhan BM (1990) Effect of germination and pure culture fermentation on phytic acid and polyphenol content of pearl millet. J Food Sci 55(4): 1180–1182.Google Scholar
  18. 18.
    Chauhan BM, Suneja N, Bhat CM (1986) Nutritional value and fatty acid composition of some high yielding varieties ofbajra. Bull Grain Technol 21(1): 441–442.Google Scholar
  19. 19.
    Oberleas D (1973) Phytates in ‘Toxicants occurring naturally in foods’. Committee on Food Protection. Fd Nutr Bd Nat Res Con pp 363–371.Google Scholar
  20. 20.
    Thompson LU, Serraino MR (1985) Effect of germination on phytic acid, protein and fat content of rapeseed. J Food Sci 50: 1200–1201.Google Scholar
  21. 21.
    Daniels DGH, Fisher N (1981) Hydrolysis of the phytate of wheat flour during bread making. Brit J Nutr 46: 1–6.PubMedGoogle Scholar
  22. 22.
    Khetarpaul N, Chauhan BM (1991a) Effect of natural fermentation on antinutrients andin vitro digestibility of starch and protein in pearl millet flour. J Sci Food Agric 55: 189–195.Google Scholar
  23. 23.
    Khetarpaul N, Chauhan BM (1991b) Sequential fermentation of pearl millet by yeasts and lactobacilli: Effect on the antinutrients andin vitro digestibility. Plant Foods Hum Nutr 41(4): 321–327.PubMedGoogle Scholar
  24. 24.
    Mahajan S (1986) Studies on the improvement of nutritional value ofbajra through fermentation. M.Sc. Thesis, Haryana Agricultural University, Hisar, India.Google Scholar
  25. 25.
    Rao PU, Deosthale YG (1982) Tannin content of pulses: Varietal differences and effects of germination and cooking. J Sci Food Agric 33: 1013–1016.Google Scholar
  26. 26.
    Kataria A, Chauhan BM, Punia D (1989) Anti-nutrients and protein digestibility (in vitro) of mung bean as affected by domestic processing. Food Chem 34: 1010–1012.Google Scholar
  27. 27.
    Dhankher N, Chauhan BM (1987) Effect of temperature and fermentation time on phytic acid and polyphenol content ofrabadi — A fermented pearl millet food. J Food Sci 52: 828–829.Google Scholar
  28. 28.
    Srivastava S (1994) Study on the effects of processing on nutrient and non-nutrient composition ofLathyrus sativus. M.Sc. Thesis, CCS Haryana Agricultural University, Hisar, India.Google Scholar
  29. 29.
    Singh U, Kherkar MS, Jambunathan R (1982) Studies on thedesi andkabuli chickpea cultivars. The levels of amylase inhibitors, levels of oligosaccharides andin vitro starch digestibility. J Food Sci 47: 510–512.Google Scholar
  30. 30.
    Mulimani VH, Supriya D (1993) Alpha-amylase inhibitor activity in sorghum grains: Effects of cooking and UV radiation. J Food Sci Technol 30(5): 321–323.Google Scholar
  31. 31.
    Haug W, Lantzsch HJ (1983) Sensitive method for the rapid determination of phytate in cereals and cereal products. J Sci Food Agric 34: 1423–1426.Google Scholar
  32. 32.
    Bernfeld P (1955) Amylases alpha and beta. In: Methods in Enzymology, Ist edn. Eds. SP Colowick and NO Kaplan Acad Press, NY p 149–159.Google Scholar
  33. 33.
    Singh U, Jambunathan R (1981) Studies ondesi andkabuli chickpea (Cicer arietinum L.) cultivars. Levels of protease inhibitors, levels of polyphenolic compounds andin vitro protein digestibility. J Food Sci 46: 1364–1367.Google Scholar
  34. 34.
    Swain T, Hillis WE (1959) The phenolic constituents ofPrunus domestica 1. The quantitative analysis of phenolic constituents. J Sci Food Agric 10: 63–68.Google Scholar
  35. 35.
    Snedecor GW and Cochran WG (1967) Statistical Methods. Oxford IBH Publishing Co, New Delhi, India.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Alka Sharma
    • 1
  • A. C. Kapoor
    • 1
  1. 1.Department of Foods and NutritionCCS Haryana Agricultural UniversityHisarIndia

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