Plant Cell Reports

, Volume 16, Issue 7, pp 490–494 | Cite as

In vitro multiplication ofVanilla planifolia using axillary bud explants

  • P. S. George
  • G. A. Ravishankar


A clonal propagation method has been developed for efficient multiplication ofVanilla planifolia. Multiple shoots were developed from axillary bud explants using semi-solid Murashige and Skoog (MS) medium supplemented with N6-benzyladenine (BA, 2 mg l−1) and α-naphthaleneacetic acid (NAA, 1 mg l−1). The multiple shoots were transferred to agitated liquid MS medium with BA at 1 mg l−1 and NAA at 0.5 mg l−1 for 2–3 weeks, and subsequently cultured on semi-solid medium. Using this method, an average of 42 shoots were obtained from a single axillary bud explant over a period of 134 days. Use of an intervening liquid medium has been found to enhance multiplication of shoots inV. planifolia.

Key words

Vanilla planifolia Axillary bud Liquid media Multiple shoots Plantlets 





Duncan's multiple-range test


Knudson (1946) medium


KC basal medium




Murashige and Skoog (1962) medium


MS basal medium

1/2 MSB

half-strength MSB


double-phase MS medium


liquid MS medium


semi-solid MS medium


α-Naphthaleneacetic acid


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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • P. S. George
    • 1
  • G. A. Ravishankar
    • 1
  1. 1.Plant Cell Biotechnology DepartmentCentral Food Technological Research InstituteMysoreIndia

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