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Plant Cell Reports

, Volume 16, Issue 7, pp 490–494 | Cite as

In vitro multiplication ofVanilla planifolia using axillary bud explants

  • P. S. George
  • G. A. Ravishankar
Article

Abstract

A clonal propagation method has been developed for efficient multiplication ofVanilla planifolia. Multiple shoots were developed from axillary bud explants using semi-solid Murashige and Skoog (MS) medium supplemented with N6-benzyladenine (BA, 2 mg l−1) and α-naphthaleneacetic acid (NAA, 1 mg l−1). The multiple shoots were transferred to agitated liquid MS medium with BA at 1 mg l−1 and NAA at 0.5 mg l−1 for 2–3 weeks, and subsequently cultured on semi-solid medium. Using this method, an average of 42 shoots were obtained from a single axillary bud explant over a period of 134 days. Use of an intervening liquid medium has been found to enhance multiplication of shoots inV. planifolia.

Key words

Vanilla planifolia Axillary bud Liquid media Multiple shoots Plantlets 

Abbreviations

BA

N6-benzyladenine

DMRT

Duncan's multiple-range test

KC

Knudson (1946) medium

KCB

KC basal medium

Kn

kinetin

MS

Murashige and Skoog (1962) medium

MSB

MS basal medium

1/2 MSB

half-strength MSB

MS-D

double-phase MS medium

MS-L

liquid MS medium

MS-S

semi-solid MS medium

NAA

α-Naphthaleneacetic acid

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References

  1. Borkird C, Sink KC (1983) Medium components for shoot cultures of chlorophyll-deficient mutants ofPetunia inflata. Plant Cell Rep 2:1–4Google Scholar
  2. Davidonis G, Knorr D (1991) Callus formation and shoot regeneration inVanilla planifolia. Food Biotechnol 5:59–66Google Scholar
  3. Duncan DB (1955) Multiple range and multipleF test. Biometrics 11:1–42Google Scholar
  4. Goodenough DR (1982)Vanilla, vanillin and vanillin derivatives. Bakers Dig 56:8–10Google Scholar
  5. Gu Z, Arditti J, Nyman LP (1987)Vanilla planifolia: callus induction and plantlet production in vitro. Lindleyana 2:48–52Google Scholar
  6. Knorr D, Caster C, Dorneburg H, Dorn R, Graf S, Havkin-Frenkel D, Podstolski A, Werrman U (1993) Biosynthesis and yield improvement of food ingredients from plant cell and tissue cultures. Food Technol 12:57–63Google Scholar
  7. Knudson L (1946) A new nutrient solution for the germination of orchid seed. Am Orchid Soc Bull 15:214–217Google Scholar
  8. Kononowicz H, Janick J (1984) In vitro propagation ofVanilla planifolia. Hort Science 19:58–59Google Scholar
  9. Krikorian AD, Kann RP (1979) Clonal propagation of daylilies. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture. Ohio State University Press, Columbus, pp 835–836Google Scholar
  10. Molnar GY (1987) A new patented method for mass propagation of shoot cultures. Acta Hort 212:125–130Google Scholar
  11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  12. Philip VJ, Nainar SAZ (1986) Clonal propagation ofVanilla planifolia (Salisb) Ames using tissue culture. J Plant Physiol 122:211–215Google Scholar
  13. Simmonds JA, Cumming BG (1976) Propagation ofLilium hybrids. II. Production of plantlets from bulb scale callus cultures for increased propagation rates. Sci Hort 5:161–170Google Scholar
  14. Stafford A (1991) The manufacture of food ingredients using plant cell and tissue cultures. Trends Food Sci Technol 2:116–122Google Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • P. S. George
    • 1
  • G. A. Ravishankar
    • 1
  1. 1.Plant Cell Biotechnology DepartmentCentral Food Technological Research InstituteMysoreIndia

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