Plant Cell Reports

, Volume 16, Issue 7, pp 490–494

In vitro multiplication ofVanilla planifolia using axillary bud explants

  • P. S. George
  • G. A. Ravishankar
Article

DOI: 10.1007/BF01092772

Cite this article as:
George, P.S. & Ravishankar, G.A. Plant Cell Reports (1997) 16: 490. doi:10.1007/BF01092772

Abstract

A clonal propagation method has been developed for efficient multiplication ofVanilla planifolia. Multiple shoots were developed from axillary bud explants using semi-solid Murashige and Skoog (MS) medium supplemented with N6-benzyladenine (BA, 2 mg l−1) and α-naphthaleneacetic acid (NAA, 1 mg l−1). The multiple shoots were transferred to agitated liquid MS medium with BA at 1 mg l−1 and NAA at 0.5 mg l−1 for 2–3 weeks, and subsequently cultured on semi-solid medium. Using this method, an average of 42 shoots were obtained from a single axillary bud explant over a period of 134 days. Use of an intervening liquid medium has been found to enhance multiplication of shoots inV. planifolia.

Key words

Vanilla planifolia Axillary bud Liquid media Multiple shoots Plantlets 

Abbreviations

BA

N6-benzyladenine

DMRT

Duncan's multiple-range test

KC

Knudson (1946) medium

KCB

KC basal medium

Kn

kinetin

MS

Murashige and Skoog (1962) medium

MSB

MS basal medium

1/2 MSB

half-strength MSB

MS-D

double-phase MS medium

MS-L

liquid MS medium

MS-S

semi-solid MS medium

NAA

α-Naphthaleneacetic acid

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • P. S. George
    • 1
  • G. A. Ravishankar
    • 1
  1. 1.Plant Cell Biotechnology DepartmentCentral Food Technological Research InstituteMysoreIndia
  2. 2.Department of BotanySt. Joseph's CollegeKeralaIndia

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