Four new high-lysine barley mutants, the variety ‘Lysimax’, with the high-lysine genelys3a and the mutants mother variety ‘Sultan’ were grown in a field trial in 1993 at Risø, Denmark. Mutants 609, 1242, 1385 and 1405 yielded in the range of 89 to 98 percent and cv ‘Lysimax’ yielded 102 percent of cv ‘Sultan’ (100 percent). One-thousand kernel weights for the mutants were in the range of 87 to 97 percent and cv ‘Lysimax’ 83 percent of cv ‘Sultan’ (100 percent). Protein contents of the mutants were slightly higher, in the range of 13.2 to 13.6 percent, than of cv ‘Sultan’ (13.1 percent) and ‘Lysimax’ which had a protein content of 12.6 percent. Fat content was higher in ‘Lysimax’ and in the mutants except for mutant 1385 than in cv ‘Sultan’ while dietary fibre contents of the barleys were similar. The levels of β-glucans and starch were usually lower in ‘Lysimax’ and in the mutants. The highest lysine levels: 4.6, 4.0 and 3.7 g/16 g N occurred in cv ‘Lysimax’ and mutants 609 and 1405 compared to 3.3, 3.3 and 3.2 for cv ‘Sultan’ and mutants 1242 and 1385, respectively. Mutants 609 and 1405 and cv ‘Lysimax’ also had higher levels of threonine, histidine and valine. The increased lysine contents resulted in large, at most 20 percent, increases in biological value; 88.8, 81.7 and 78.3 percent for cv ‘Lysimax’ and mutants 609 and 1405 compared to 74.2 percent for cv ‘Sultan’. True protein digestibilities and energy digestibilities were slightly lower in ‘Lysimax’ than in ‘Sultan’, 5.3 and 4.3 percentage units, respectively. It is concluded that the development of high-lysine barley varieties is very beneficial for meeting the requirements of indispensable amino acids for humans and monogastric animals. In addition, nitrogen excretion into the environment is drastically reduced due to the higher biological values of the mutants.
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AOAC (1984) Official Methods of Analysis, 14th ed. Washington, DC: Association of Official Analytical Chemists.
Bach Knudsen KE, Li B (1991) Determination of oligosaccharides in protein-rich feedstuffs by gas-liquid chromatography and high-performance liquid chromatography. J Agric Food Chem 39: 689–694.
Bach Knudsen KE, Jensen BB, Hansen I (1993) Digestion of polysaccharides and other major components in the small and large intestine of pigs fed on diets consisting of oat fractions rich in β-D-glucan. Brit J Nutr 70: 537–556.
Bang-Olsen K, Stilling B, Munck L (1987) Breeding for yield in high-lysine barley. In: Yasuda S, Konishi T (eds), Barley genetics V. Proceedings of the 5th international barley genetics symposium. Okyama, Japan: Maruzen, pp 865–870.
Bang-Olsen K, Stilling B, Munck L (1991) The feasibility of high lysine barley breeding — a summary. In: Munck L (ed), Barley genetics VI. Proceedings of the 6th international barley genetics symposium Helsingberg, Sweden. Copenhagen: Munksgaard International, pp 433–438.
Bansal HC, Srisvastava KNK, Eggum BO, Metha LS (1977) Nutritional evaluation of high protein genotypes of barley. J Sci Food Agric 28: 157–160.
Bech-Andersen S (1991) Determination of tryptophan with HPLC after alkaline hydrolysis in autoclave using α-methyl-tryptophan as internal standard. Acta Agric Scand 41: 305–309.
Di fonzo N, Stanca AM (1977) EMS derived barley mutants with increased lysine content. Genetica Agraria 31: 401–409.
Doll H (1980) A nearly non-functional mutant allele of the storage protein locusHor-2 in barley. Hereditas 93: 217–222.
Doll H, Køie B, Eggum BO (1974) Induced high-lysine mutants of barley. Rad Bot 14: 73–80.
Eggum BO (1973) A study of certain factors influencing protein utilization in rats and pigs. Copenhagen: National Institute of Animal Science, Publ 406, 173p.
Eggum BO (1977) Nutritional aspects of cereal proteins. In: Muhammed A, Aksel R, von Borstel RC (eds) Genetic diversity in plants. New York: Plenum, pp 349–369.
Haastrup Pedersen L, Ph.D. thesis (1987) Development of screening methods for evaluation of starch structure and synthesis in barley. Lyngby, Denmark: Danish Academy of Technical Sciences.
Hagberg A, Karlsson KE (1969) Breeding for high protein and quality in barley. In: New approaches to breeding for improved plant protein. Vienna, Austria. STI/PUB/212.
Hansen I, Larsen T, Bach Knudsen KE, Eggum BO (1991) Nutrient digestibilities in ingredients fed alone or in combinations. Brit J Nutr 66: 27–35.
Jensen J, Doll H (1979) Gene symbols for barley high-lysine mutants. Barley Genetics Newsletter 9: 33–37.
Mason VC, Bech-Anderson S, Rudemo M (1980) Hydrolysate preparation for amino acid determinations in feed constituents. Z Tierphysiol Tierernährg Futtermittelkde 43: 146–164.
McCleary BV, Glennie-Holmes MJ (1985) Enzymatic quantification of (1–3), (1–4)-β-D-glucan in barley and malt. J Inst Brewing 91: 285–295.
McIntosh GH, Le Leu RK, Kerry A, Goldring M (1993) Barley grain for human food use. Food Aust 45: 392–394.
Munck L (1972) Improve of nutritional value in cereal. Hereditas 72: 1–128.
Munck L (1992) The case of high-lysine barley breeding. In: Shewry PR (ed), Barley: genetics, biochemistry, molecular biology and biotechnology. Wallingford Oxon, UK: CAB International, pp 573–601.
Munck L, Karlson KE, Hagberg A, Eggum BO (1970) Gene for improved nutritional value in barley seed protein. Science 168: 985–987.
Newman CW, Eslick RF, Rasmunson RC (1974) Effect of barley variety on protein quality and nutritional value for rats. J Anim Sci 38: 71–75.
Newman CW, Eslick RF, Moss BR, El-Negoumy AM (1977) ‘Hiproly’ barley as a source of protein and amino acids for growing-finishing pigs. Nutr Rep Int 15: 383–390.
Newman CW, El-Negoumy AM, Eslick RF (1978) Replacing soy protein in swine diets with high-protein barley and amino acids. J Anim Sci 46: 161–166.
Newman CW, Øverland M, Newman RK, Bang-Olsen K, Pedersen B (1990) Protein quality of new high-lysine barley derived from Risø 1508. Can J Anim Sci 70: 279–285.
Newman RK, Newman CS (1991) Barley as a food grain. Cer Foods World 36: 800–805.
Oram RN, Doll H (1981) Yield improvement in high lysine barley. Aust J Agric Res 32: 425–434.
SAS (1988) SAS/GRAPH® User's Guide (Release 6.03). Cary, NC, USA: SAS Inst Inc.
SAS (1988) SAS/STAT® User's Guide (Release 6.03). Cary, NC, USA: SAS Inst Inc.
Sauer WC, Eggum BO, Jacobsen I (1979) The influence of level and source of fibre on protein utilization in rats. Arch Tierernähr 29: 533–540.
Shewry PR, Field JM, Kirkman MA, Faulks AJ, Miflin BJ (1980) The extraction, solubility and characterization of two groups of barley storage polypeptides. J Exp Bot 31: 393–407.
Steel RGD, Torrie JH (1980) Principles and procedures of statistics a biometrical approach, 2nd ed. New York: McGraw-Hill Book Company.
Stoldt W (1952) Vorschlag zur Vereinheitlichung der Fettbestimmung in Lebensmitteln. Fette, Seifen, Anstrichmittel 54: 206–207.
Tallberg A (1981) Protein and lysine content in high-lysine, a double-recessive barley. II. Combinations between mutant 7 and ‘2 Hiproly’ back cross. Hereditas 94: 261–268.
Tallberg A, Eggum BO (1981) The nutritional value of high-lysine barley genotypes. Plant Foods Hum Nutr 31: 151–161.
Tallberg A, Eggum BO (1986) Grain yields and nutritional qualities of some high-lysine barley hybrids. J Cer Sci 4: 345–352.
Theander O, Westerlund EA (1986) Studies on dietary fiber. 3. Improved procedures for analysis of dietary fiber. J Agric Food Chem 34: 330–336.
WHO (1985) Energy and protein requirements. Report of a Joint FAO/WHO/UNU Expert Consultation. Geneva: World Health Organization, Techn Rep Ser 724, 206p.
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Gabert, V.M., Brunsgaard, G., Eggum, B.O. et al. Protein quality and digestibility of new high-lysine barley varieties in growing rats. Plant Food Hum Nutr 48, 169–179 (1995). https://doi.org/10.1007/BF01088313
- Amino acids
- Biological value