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Multiple shoot formation from normal and malformed somatic embryo explants of Eastern redbud (Cercis canadensis L.)


A procedure for multiple shoot formation from somatic embryo explants of Eastern redbud (Cercis canadensis L.) cultured on DKW medium containing benzyladenine (BA) and thidiazuron (TDZ) was developed. TDZ in combination with BA produced more shoots than either treatment alone. The highest number of shoots (3.3 to 3.4 shoots per explant) was obtained from partially desiccated and wounded explants treated with a combination of 5 or 10 μM BA and 0.5 or 1.0 μM TDZ for 20 days before being transferred to the same medium without TDZ. The number of shoots formed was increased from 1.5 to 3.2 shoots per explant by cutting through the cotyledonary node prior to culture. In addition, the frequency of explants forming shoots was increased by desiccation of somatic embryo explants to approximately 50% moisture and by using somatic embryos with two well formed cotyledons as explants.

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Completely randomized design


Driver and Kuniyuki medium


Least significant differences




  1. Bailey MA, Boerma HR, Parrott WA (1993) Plant Sci 93: 117–120.

  2. Baker CM, Wetzstein HY (1992) Plant Cell Rep 11: 71–75.

  3. Buchheim J, Colbum S, Ranch L (1989) Plant Physiol 89:768–775.

  4. Chengalrayan K, Sathaye SS, Hazra S (1994) Plant Cell Rep 13: 578–581.

  5. Copeland LO, McDonald MB (1995) Principles of seed science and technology. Chapman and Hall, New York. pp. 181–220.

  6. Dinesh Kumar V, Kirti PB, Sachan JKS, Chopra VL (1994) Plant Cell Rep 13: 468–472.

  7. Distabanjong K, Geneve RL (1996) Plant Cell Tiss Org Cult in Press.

  8. Durham RE, Parrott WA (1992) Plant Cell Rep 11: 122–125.

  9. Geneve RL (1991) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry: Trees III. Vol. 16. Springer-Verlag, New York. pp. 142–151.

  10. Geneve RL, Kester ST (1990) Plant Cell Tiss Org Cult 22: 71–76.

  11. Kermode AR (1990) Critical Reviews Plant Sci 9: 155–195.

  12. Lai F, McKersie BD (1994) Plant Cell Tiss Org Cult 37: 151–158.

  13. Lazzeri PA, Hildebrand DF, Collins GB (1987) Plant Cell Tiss Org Cult 10: 197–208.

  14. Lazzeri PA, Hildebrand DF, Sunega J, Williams EG, Collins GB (1988) Plant Cell Rep 7: 517–520.

  15. McClean P, Grafton KF (1989) Plant Sci 60: 117–122.

  16. Mohamed MF, Read PE, Coyne DP (1992) J Amer Soc Hort Sci 117: 668–672.

  17. Parrott WA, Dryden G, Vogt S, Hildebrand DF, Collins GB, Williams EG (1988) In Vitro Cell Dev Biol 24: 817–820.

  18. Parrott WA, Durham RE, Bailey MA (1995) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry series, Vol. 31,Somatic Embryogenesis and Synthetic Seed II. Springer-Verlag, Berlin, pp 199–227.

  19. Roberts DR, Flinn BS, Webb DT, Webster FB, Sutton BCS (1990) Physiol Plant 78: 355–360.

  20. Sagare AP, Suhasini K, Krishnamurthy KV (1993) Plant Cell Rep 12: 652–655.

  21. SAS Institute Inc. (1989) SAS/STAT User's Guide, Version 6, Fourth Edition, SAS Institute Inc., Cary, NC.

  22. Senaratna T, McKersie BD, Bowley SR (1989) Plant Sci 65: 253–259.

  23. Tremblay L, Tremblay FM (1991) Plant Sci 77: 233–242.

  24. Trigiano RN, Beaty RM, Graham ET (1988) Plant Cell Rep 7: 148–150.

  25. Wetzstein HY, Baker CM (1993) Plant Sci 92: 81–89.

  26. Wright MS, Launis KL, Novitaky R, Duesing IH, Harms CT (1991) In Vitro Cell Dev Biol 27P: 153–157.

  27. Yusnita S, Geneve RG, Kester ST (1990) J Environ Hort 8:177–179.

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Correspondence to Robert L. Geneve.

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Communicated by W A. Parrott

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Distabanjong, K., Geneve, R.L. Multiple shoot formation from normal and malformed somatic embryo explants of Eastern redbud (Cercis canadensis L.). Plant Cell Reports 16, 334–338 (1997).

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  • Somatic Embryo
  • Multiple Shoot
  • Shoot Formation
  • Benzyladenine
  • Thidiazuron