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Multiple shoot formation from normal and malformed somatic embryo explants of Eastern redbud (Cercis canadensis L.)

Abstract

A procedure for multiple shoot formation from somatic embryo explants of Eastern redbud (Cercis canadensis L.) cultured on DKW medium containing benzyladenine (BA) and thidiazuron (TDZ) was developed. TDZ in combination with BA produced more shoots than either treatment alone. The highest number of shoots (3.3 to 3.4 shoots per explant) was obtained from partially desiccated and wounded explants treated with a combination of 5 or 10 μM BA and 0.5 or 1.0 μM TDZ for 20 days before being transferred to the same medium without TDZ. The number of shoots formed was increased from 1.5 to 3.2 shoots per explant by cutting through the cotyledonary node prior to culture. In addition, the frequency of explants forming shoots was increased by desiccation of somatic embryo explants to approximately 50% moisture and by using somatic embryos with two well formed cotyledons as explants.

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Abbreviations

ABA:

abscisic acid

BA:

benzyladenine

CRD:

Completely randomized design

DKW:

Driver and Kuniyuki medium

LSD:

Least significant differences

TDZ:

thidiazuron

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Author information

Correspondence to Robert L. Geneve.

Additional information

Communicated by W A. Parrott

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Distabanjong, K., Geneve, R.L. Multiple shoot formation from normal and malformed somatic embryo explants of Eastern redbud (Cercis canadensis L.). Plant Cell Reports 16, 334–338 (1997). https://doi.org/10.1007/BF01088292

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Keywords

  • Somatic Embryo
  • Multiple Shoot
  • Shoot Formation
  • Benzyladenine
  • Thidiazuron