Biotechnology Letters

, Volume 10, Issue 6, pp 389–392 | Cite as

Improved stability of the continuous production of acetone-butanol by Clostridium acetobutylicum in a two-stage process

  • C. Godin
  • J. M. Engasser


A stable continuous culture has been maintained for 30 days at a high 20 g/l solvent concentration. This substantial increase in the stability of the continuous culture ofClostridium acetobutylicum at the maximal solvent level was achieved by using a two-stage process with a dilution rate of 0.1 h−1 in the first fermentor and 0.04 h−1 in the second fermentor. The two-stage continuous fermentation allows an optimal growth of cells and induction of solvent metabolism in the first stage, and a maximal production yield of solvents in the second stage.


Fermentation Production Yield Clostridium Optimal Growth Dilution Rate 
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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • C. Godin
    • 1
  • J. M. Engasser
    • 1
  1. 1.Laboratoire des Sciences du Génie ChimiqueCNRS-ENSIC-ENSAIA Institut National Polytechnique de LorraineNancy CedexFrance

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