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Electron microscopic demonstration of two types of mitochondria with different affinities for lead

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Incubation of glutaraldehyde-fixed, non-frozen tissue slices of mouse seminal vesicle, ventral prostate and small intestine in the media described by Hugon & Borgers (1966) and by Mayaharaet al. (1967) for the cytochemical demonstration of alkaline phosphatase resulted in the deposition of lead along smooth muscle mitochondrial membranes but not along epithelial mitochondrial membranes. Control studies using boiled tissues; media lacking substrate; inhibitors such as L-cysteine, EDTA,N-ethyl maleimide andp-chloromercuribenzoate; and tissues incubated in full media after fixation, embedding and sectioning, showed that the reaction in muscle mitochondria was non-enzymatic. It was concluded that the phospholipid component of muscle mitochondrial membranes differed from that of epithelial mitochondrial membranes.

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  1. NBarrnett, R. B. (1964). Localisation of enzymatic activity at the fine structural level.Jl R. microsc. Soc. 83, 143–51.

  2. Bourne, G. H. (1964).Cytology and Cell Physiology, 3rd Ed., p. 377. New York: Academic Press.

  3. Bradley, D. E. (1965). InTechniques for Electron Microscopy, 2nd Ed., p. 58. (ed. D. H. Kay). Oxford: Blackwell.

  4. Burstone, M. (1962).Enzyme Histochemistry and its Applications in the Study of Neoplasms, p. 160. New York: Academic Press.

  5. de Robertis, E. D. P., Nowinski, W. W. &Saez, F. A. (1960).General Cytology, 3rd Ed., p. 169. Philadelphia: Saunders.

  6. Epstein, M. &Holt, S. J. (1963). The localisation by electron microscopy of HeLa cell surface enzymes splitting ATP.J. Cell Biol. 19, 325–36.

  7. Glauert, A. M. (1965). InTechniques for Electron Microscopy, 2nd Ed., p. 166. (ed. D. H. Kay). Oxford: Blackwell.

  8. Goldfischer, S., Essner, E. &Novikoff, A. B. (1963). The localisation of phosphatase activities at the level of ultrastructure.J. Histochem. Cytochem. 12, 72–95.

  9. Green, D. E. (1964). The mitochondrion.Sci. Amer. 210, 63–71.

  10. Green, D. E. &MacLennan, D. H. (1967). InMetabolic Pathways, Vol. I. 3rd Ed., p. 47. (ed. D. M. Greenberg), New York: Academic Press.

  11. Holt, S. J. (1959). Factors governing the validity of staining methods for enzymes and their bearing upon the Gomori acid phosphatase technique.Expl Cell Res. (Suppl.)7, 1–27.

  12. Hugon, J. &Borgers, M. (1966a). A direct lead method for the electron microscopic visualisation of alkaline phosphatase activity.J. Histochem. Cytochem. 14, 429–31.

  13. Hugon, J. &Borgers, M. (1966b). Ultrastructural localisation of alkaline phosphatase activity in the absorbing cells of the duodenum of mouse.J. Histochem. Cytochem. 14, 629–40.

  14. Hugon, J., Borgers, M. &Loni, M. C. (1967). Alkaline phosphatase activity in HeLa cells.J. Histochem. Cytochem. 15, 417–18.

  15. Mayahara, H., Hirano, H., Saito, T. &Ogawa, K. (1967). The new lead citrate method for the ultracytochemical demonstration of non-specific alkaline phosphatase (orthophosphoric monoester phosphohydrolase).Histochemie 11, 88–96.

  16. Ogawa, K. &Barrnett, R. B. (1964). Electron histochemical examination of oxidative enzymes and mitochondria.Nature, Lond. 203, 724–26.

  17. Pearse, A. G. E. (1960).Histochemistry: theoretical and applied 2nd Ed., p. 384. London: Churchill.

  18. Roodyn, D. B. (1967).Enzyme Cytology, p. 103. London: Academic Press.

  19. Sabatini, D. D., Bensch, K. &Barrnett, R. (1963). Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.J. Cell Biol. 17, 19–58.

  20. Smith, R. E. &Farquhar, M. G. (1965). Preparation of non-frozen sections for electron microscope cytochemistry.Scient. Instrum. News. 10, 13–18.

  21. Sommers, A. J. (1954). The determination of acid and alkaline phosphatase usingp-nitrophenyl phosphate as substrate.Am. J. Med. Tech. 20, 244–53.

  22. Stoeckenius, W. (1962). InThe Interpretation of Ultrastructure, p. 349. (ed. R. J. C. Harris). London: Academic Press.

  23. Thomas, R. S. &Greenawalt, J. W. (1968). Microincineration, electron microscopy, and electron diffraction of calcium phosphate-loaded mitochondria.J. Cell Biol. 39, 55–76.

  24. Wachstein, M. &Meisel, E. (1957). Histochemistry of hepatic phosphatases at a physiological pH with special reference to the demonstration of bile canaliculi.Am. J. clin. Path. 27, 13–23.

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Wilson, P.D. Electron microscopic demonstration of two types of mitochondria with different affinities for lead. Histochem J 1, 405–416 (1969). https://doi.org/10.1007/BF01086982

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  • Smooth Muscle
  • Alkaline Phosphatase
  • Small Intestine
  • Mitochondrial Membrane
  • Seminal Vesicle