Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Application of flowcell HPLC-FTIR technique to the analysis of benzoylated saccharides and compounds with similar polarity

Anwendung der HPLC-FTIR-Kopplung zur Analyse benzoylierter Saccharide und Verbindungen mit ähnlicher Polarität

  • 59 Accesses

  • 5 Citations

Zusammenfassung

Der Einsatz eines Fourier-Transform-Infrarotspektrometers (FTIR) in der Verbindung mit der HPLC ermöglicht die simultane Detektion bei mehreren Wellenzahlen. Wegen der großen Eigenabsorption der bei RP-Chromatographie meistens benötigten Fließmittel ist eine IR-Detektion dort nicht sinnvoll. Das ternäre, in einem weiten Bereich variable Elutionsmittelgemisch Isooctan/Diethylether/Acetonitril bietet gute UV- und IR-Detektionsmöglichkeiten bei der Verwendung von Kieselgelsäulen. Es werden HPLC-FTIR-Analysen von Reinsubstanzen (PHB-Ester) und für benzoylierte Extrakte (Sojamehl, Hautbräunungsmittel) gezeigt.

Summary

Fourier transform infrared (FTIR) spectrometry in combination with liquid chromatography provides nonspecific detection and highly specific multichannel analysis simultaneously. Standard reversed-phase eluents are incompatible with IR-flow-cell detection because of their strong self-absorptions. The ternary solvent system isooctane/diethyl ether/acetonitrile, which exhibits adequately adjustable polarity, has already revealed its potential in the separation of derivatized food extracts on silica columns using UV detection. In the HPLC-FTIR analyses reported in this investigation different compositions of this ternary solvent system were applied to the analysis of an artificial mixture, of benzoylated soy flour and benzoylated tanning product.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Kuehl DT, Griffiths PR (1980) Anal Chem 52:1394–1399

  2. 2.

    Jinno K, Fujimoto C (1983) Chromatographia 17:259–261

  3. 3.

    Combellas C, Bayart H (1983) J Chromatogr 259:211–225

  4. 4.

    Fölster U, Herres W (1983) Farbe + Lack 89:417–420

  5. 5.

    Johnson CC, Taylor LT (1983) Anal Chem 55:436–438

  6. 6.

    Brown RS, Amateis PG, Taylor LT (1984) Chromatographia 18:396–400

  7. 7.

    Jinno K, Fujimoto C, Nakaniski S (1985) Chromatographia 20:279–282

  8. 8.

    Galensa R (1983) Z Lebensm Unters Forsch 176:417–420

  9. 9.

    Galensa R (1984) Z Lebensm Unters Forsch 178:199–202

  10. 10.

    Siewek F, Galensa R (1984) J Chromatogr 294:385–389

  11. 11.

    Galensa R, Schuster B (1985) Dtsch Lebensm Rundsch 81:273–275

Download references

Author information

Correspondence to Rudolf Galensa.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Galensa, R., Landt, K. & Herres, W. Application of flowcell HPLC-FTIR technique to the analysis of benzoylated saccharides and compounds with similar polarity. Z Lebensm Unters Forch 185, 36–38 (1987). https://doi.org/10.1007/BF01083338

Download citation

Keywords

  • Chromatography
  • Fourier
  • HPLC
  • Saccharide
  • Fourier Transform