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Growth of human acute myeloblastic leukemic (AML) cells in vitro

In vitro Wachstum der menschlichen akut myeloblastischen (AML) Zellen

Zusammenfassung

In einem Plasma-Gerinnselsystem bilden normale menschliche Vorläuferzellen der Hämatopoese und leukämische Zellen bei myeloischer Leukämie Kolonien und Cluster. Anzahl und Zusammensetzung der gebildeten Kolonien sind den Verhältnissen vergleichbar, die von anderen Systemen her bekannt sind (Agar), welche gewöhnlich für diese Untersuchungen verwendet werden. Das hier vorgestellte Plasma-Gerinnselsystem hat den Vorteil, daß die Zellen der gebildeten Kolonien in situ morphologisch und zytochemisch charakterisiert werden können. Diese Ergebnisse werden ebenfalls verglichen mit dem Wachstum leukämischer Zellen in Suspensionskulturen innerhalb konventioneller und modifizierter Marbrook-Flaschen. Die hier vorgelegten Studien belegen, daß das Marbrook-System für Kulturen, die bis zu 7 Tage inkubiert werden, keine Vorteile besitzt.

Summary

Human normal and granulocytic leukemic progenitor cells produce colonies and clusters when cultured in the plasma clot system in vitro. The number of colonies formed and their cellular composition was comparable to that reported for the system (soft agar) usually used for these studies. The plasma clot system has the advantage of permitting in situ morphologic and cytochemical characterization of the cells within the colonies. A comparison was also made of the growth of leukemic cells in suspension cultures placed within conventional and modified Marbrook flasks. These studies demonstrated no advantage for the Marbrook system in cultures incubated for up to 7 days.

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Correspondence to Dr. H. D. Preisler.

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Preisler, H.D., Christoff, G. & Epstein, C. Growth of human acute myeloblastic leukemic (AML) cells in vitro. Blut 38, 35–45 (1979). https://doi.org/10.1007/BF01082926

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Schlüsselwürter

  • Akute Leukämien
  • Plasma-Gerinnsel-Kultur

Key words

  • Acute leukemia
  • Plasma clot culture