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Die Bildung zytoplasmatischer Vakuolen phagozytierender neutrophiler Granulozyten während Sauerstoff- und Stickstoff-Begasung im in vitro-Versuch

Formation of cytoplasmic vacuoles of phagocytizing polymorphonuclear leukocytes in O2- and N2-atmosphere in in vitro-experiments

Summary

During O2 and N2 gasification in vitro, neutrophilic granulocytes ingest killed staphylococci with equal intensity. O2-gassed neutrophilic granulocytes form significantly more cytoplasmic vacuoles than N2-gassed neutrophilic granulocytes in absolute terms and in relation to the number of ingested staphylococci. The cytoplasmic vacuoles of O2-gassed neutrophilic granulocytes occupy a larger surface in smear preparations than those of N2-gassed neutrophilic granulocytes.

The results make it probable that the formation of cytoplasmic vacuoles in neutrophilic granulocytes is an O2-dependent process. This interpretation is in agreement with concepts on the peroxidation of membrane lipids and membrane destabilization. It is consistent with the process of membrane fusion and thus the formation of cytoplasmic vacuoles, which are to be regarded as phagolysosomes.

Zusammenfassung

Neutrophile Granulozyten ingestieren während O2- und N2-Begasung in vitro gleich intensiv abgetötete Staphylokokken. O2-begaste neutrophile Granulozyten bilden absolut und bezogen auf die Zahl ingestierter Staphylokokken signifikant mehr zytoplasmatische Vakuolen als N2-begaste neutrophile Granulozyten. Die gebildeten zytoplasmatischen Vakuolen O2-begaster neutrophiler Granulozyten nehmen in Ausstrichpräparaten eine größere Fläche ein als die von N2-begasten neutrophilen Granulozyten.

Die Ergebnisse machen wahrscheinlich, daß die Bildung von zytoplasmatischen Vakuolen in neutrophilen Granulozyten ein O2-abhängiger Prozeß ist. Diese Deutung steht in Übereinstimmung mit Vorstellungen über Peroxidation von Membranlipiden, Membrandestabilisierung, mit dem Vorgang der Membranfusion und somit der Bildung von Phagolysosomen, die in Form von zytoplasmatischen Vakuolen erkennbar sind.

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Eschenbach, C., Leiter, J. Die Bildung zytoplasmatischer Vakuolen phagozytierender neutrophiler Granulozyten während Sauerstoff- und Stickstoff-Begasung im in vitro-Versuch. Blut 42, 13–21 (1981). https://doi.org/10.1007/BF01080259

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Key words

  • Polymorphonuclear leukocytes
  • Formation of cytoplasmic vacuoles
  • Phagocytosis
  • Bactericidal capacity

Schlüsselwörter

  • Neutrophile Granulozyten
  • Bildung zytoplasmatischer Vakuolen
  • Phagozytose
  • Bakterizidie