Biotechnology Letters

, Volume 8, Issue 12, pp 843–848 | Cite as

Production of corrinoids including vitamin B-12 byMethanosarcina barkeri growing on methanol

  • Tapan K. Mazumder
  • N. Nishio
  • M. Hayashi
  • S. Nagai


A preliminary attempt was made for producing vitamin B-12 byMethanosarcina barkeri strain Fusaro in a fed-batch culture with a methanol minimum medium. After 11 days, total methanol consumption, cell density and corrinoid concentration were 145 g/l, 8.5 g(dry cell weight)/l, and 135 mg/l (73% in supernatant) respectively. Electrophoretic separation revealed that 33% of the total corrinoids was B-12 Factor III (5-hydroxybenzimidazolylcobamide) and the remaining corrinoids were cobinamide (Factor B) and its derivatives.


Methanol Cell Density Minimum Medium Cell Weight Electrophoretic Separation 
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Copyright information

© Science and Technology Letters 1986

Authors and Affiliations

  • Tapan K. Mazumder
    • 1
  • N. Nishio
    • 1
  • M. Hayashi
    • 1
  • S. Nagai
    • 1
  1. 1.Department of Fermentation Technology, Faculty of EngineeringHiroshima UniversityHigashi-Hiroshima 724Japan

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