Molecular and Cellular Biochemistry

, Volume 127, Issue 1, pp 179–186 | Cite as

Signal transduction through the cAMP-dependent protein kinase

  • Judy L. Meinkoth
  • Arthur S. Alberts
  • Wei Went
  • Dominic Fantozzi
  • Susan S. Taylor
  • Masatoshi Hagiwara
  • Marc Montminy
  • James R. Feramisco
Protein Phosphorylation in Signal Transduction

Abstract

Temporal cellular events responsible for hormonal activation of responses mediated by the cAMP-dependent protein kinase (PKA) have been studied in living cells. By selectively perturbing molecular function of Gs, the catalytic subunit of PKA (C), or the nuclear factor CREB, in cells through microinjection of inhibitory agents specific for these molecules or activated forms of these molecules, we have obtained evidence for a requirement for the function of each of these molecules in the hormonal stimulation of cAMP-regulated genes. Moreover, by introducing fluorescently labeled PKA subunits into these cells as molecular tracers, or by immunofluorescence of C subunit, we have observed biological translocation of C subunit from the cytoplasm to the nucleus during transcriptional activation and a quenching of this by the inhibitor molecule, PKI. The implications of these cellular and molecular events in the signal transduction of hormonal responses are discussed.

Key words

protein kinases signal transduction transcription activity phosphorylation microinjection 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Judy L. Meinkoth
    • 1
    • 4
  • Arthur S. Alberts
    • 2
  • Wei Went
    • 3
  • Dominic Fantozzi
    • 3
  • Susan S. Taylor
    • 3
    • 4
  • Masatoshi Hagiwara
    • 5
  • Marc Montminy
    • 5
  • James R. Feramisco
    • 1
    • 2
    • 4
  1. 1.Department of MedicineUniversity of California at San DiegoLa JollaUSA
  2. 2.Department of PharmacologyUniversity of California at San DiegoLa JollaUSA
  3. 3.Department of ChemistryUniversity of California at San DiegoLa JollaUSA
  4. 4.Cancer CenterUniversity of California at San DiegoLa JollaUSA
  5. 5.The Clayton Foundation Laboratories for Peptide BiologyThe Salk InstituteLa JollaUSA

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