Mice with a recessive gene which reduces the number of ganglion cells of the large intestine and produces megacolon similar to Hirschsprung's disease were studied. Electrical activity of the small bowel consisted of electrical slow waves and action potentials and showed no difference between the mice with megacolon and their normal siblings. Electrical slow waves and action potentials occurred in the large intestine of both normal and abnormal mice. The principal difference between normal mice and their abnormal siblings was increased incidence of discharge of action potentials associated with uncoordinated phasic contractions superimposed upon tonic contracture of the circular muscle layer of the distal aganglionic segment in the abnormal mouse. The distended colon of the abnormal mouse and the entire length of the normal bowel showed bursts of action potentials which accompanied peristaltic waves of circular muscle contraction. During propulsive motility in the rectum, activation of the circular muscle was preceded by coordinated contraction of the longitudinal muscle only in the normal bowel. Symptoms of megacolon can be accounted for by the absence of spontaneously active inhibitory neurons from the enteric plexuses of the distal segment of the large bowel.
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Derrick EH, St. George-Grambauer BM: Megacolon in mice. J Path Bacteriol 73:569, 1957
Beilschowsky M, Schofield GC: Studies on megacolon in piebald mice. Aust J Exp Biol Med Sci 40:395, 1962
Lane PW: Association of megacolon with two recessive spotting genes in the mouse. J Hered 57:29, 1966
Kuriyama H, Osa T, Toida N: Nervous activity influencing the membrane activity of intestinal muscle. J Physiol 191:257, 1967
Wood JD: Excitation of intestinal muscle by atropine, tetrodotoxin, and Xylocaine. Am J Physiol 222:118, 1972
Wood JD: Electrical activity from single neurons in Auerbach's plexus. Am J Physiol 219:159, 1970
Provenzale L, Pisana M: Methods for recording electrical activity of the human colon in vivo. Am J Dig Dis 16:712, 1971
Christensen J, Caprilli R, Lund GF: Electric slow waves in circular muscle of cat colon. Am J Physiol 217:771, 1969
Ehrenpreis T: Hirschsprung's disease. Am J Dig Dis 16:1032, 1971
Swenson O, Rheinlander HF, Diamond I: Hirschsprung's disease: a new concept of the etiology. New Engl J Med 241:551, 1949
Ohkawa H, Prosser CL: Functions of neurons in enteric plexuses of cat intestine. Am J Physiol 222:1420, 1972
Wood JD, Mayer CJ: Patterned discharge of six different neurons within a single enteric ganglion. Pfluegers Arch 338:247, 1973
Wood JD, Marsh DR: Effects of atropine, tetrodotoxin and xylocaine on rebound excitation of guinea-pig small intestine. J Pharm Exp Ther (In press)
Mayer TC: Development of piebald spotting in mice. Dev Biol 11:319, 1965
Yntema CL, Hammond WS: The origin of intrinsic ganglia of trunk viscera from vagal neural crest in the chick embryo. J Comp Neurol 101:515, 1954
Earlman RJ: A vascular cause for aganglionic bowel. Am J Dig Dis 17:255, 1972
Hukuhara T, Siina H, Fukuda H: Effects of destruction of intramural ganglion cells on the motility of the ileocecocolic region. Jap J Physiol 15:648, 1965
Shepherd JJ, Wright PG: The application of studies in vitro to the management of Hirschsprung's disease and megacolon in adults. Am J Dig Dis 13:434, 1968
De Villiers DR: Ischaemia of the colon: an experimental study. Br J Surg 53:497, 1966
Welsh JH, JE Hyde: Acetylcholine content of the myenteric plexuses and resistance to anoxia. Proc Soc Exp Biol Med 55:256, 1944
Kyi JKK, Daniel EE: The effects of ischemia on intestinal nerves and electrical slow waves. Am J Dig Dis 15:959, 1970
Szurszewski J, Steggerda FR: The effect of hypoxia on the electrical slow wave of the canine small intestine. Am J Dig Dis 13:168, 1968
Supported by National Science Foundation GB-31292 and Kansas University Medical Center General Research Support Grant RR-05973.
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Wood, J.D. Electrical activity of the intestine of mice with hereditary megacolon and absence of enteric ganglion cells. Digest Dis Sci 18, 477–488 (1973). https://doi.org/10.1007/BF01076598
- Ganglion Cell
- Circular Muscle
- Normal Bowel
- Tonic Contracture