Molecular and Cellular Biochemistry

, Volume 149, Issue 1, pp 127–130

Preliminary crystallization studies of calmodulin-dependent protein phosphatase (calcineurin) from bovine brain

  • K. Balendiran
  • Yingchun Tan
  • Rajendra K. Sharma
  • Krishna H. M. Murthy
Article

DOI: 10.1007/BF01076570

Cite this article as:
Balendiran, K., Tan, Y., Sharma, R.K. et al. Mol Cell Biochem (1995) 149: 127. doi:10.1007/BF01076570

Abstract

Calcineurin is a serine/threonine protein phosphatase which catalyzes the hydrolysis of both phosphoseryl/phosphothreonyl and phosphotyrosyl proteins as well as low molecular weight compounds such as p-nitrophenyl phosphate. It is a hetero-dimeric protein consisting of a 60 kDa A chain and 19 kDa B chain. Calcineurin A is organized into functionally distinct domains such as a catalytic domain, a calcineurin B binding domain, a calmodulin-binding domain, and an inhibitory domain. Calcineurin B has four EF-hand calcium binding domains with a secondary structure that is homologous to calmodulin but its metal binding properties are more similar to troponin-C. The N-terminal myristoyl group of calcineurin B might play a role in the interaction between subunits A and B during phosphorylation/dephosphorylation processes. Crystals of size 0.125×0.07×0.03 mm and 0.7×0.03×0.02 mm have been obtained for calcineurin and the A subunit respectively. Crystals of calcineurin show strong diffraction to 5.3 Å and weak diffraction to 3.0 Å on rotating anode operated at 50 kV and 100 mA. Further work is in progress to improve the X-ray diffraction quality of these crystals and to obtain well diffracting crystals of calcineurin B.

Key words

Crystals X-ray Calcineurin A Calcineurin B Protein Phosphatase PP2B 

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • K. Balendiran
    • 1
  • Yingchun Tan
    • 2
  • Rajendra K. Sharma
    • 2
  • Krishna H. M. Murthy
    • 1
  1. 1.Fels Institute for Cancer Research and Molecular BiologyTemple University School of MedicinePhiladelphiaUSA
  2. 2.Department of Pathology and Saskatoon Cancer Center, Royal University HospitalUniversity of SaskatchewanSaskatoonCanada

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