Molecular and Cellular Biochemistry

, Volume 153, Issue 1–2, pp 161–166

In vitro andin vivo antineoplastic effects of ortrovanadate

  • Tony F. Cruz
  • Ariela Morgan
  • Weixian Min
Part II: Biochemical and Physiological Studies

Abstract

In the present study we have demonstrated that orthovanadate at concentrations of 5–10 uM is cytotoxic to proliferating cells including primary cultures and tumour cell lines. However, concentrations of up to 50 uM did not affect the viability of non-proliferating cells. The cytotoxicity appears to be dependent on the vanadium concentration rather than on the oxidation state of vanadium or the vanadium compound. Furthermore, tumour cell lines with different proliferative rates were equally sensitive to orthovanadate cytotoxicity. Although the mechanisms responsible for the cytotoxicity are not known, addition of H2O2 potentiated orthovanadate cytotoxicity suggesting that hydroxyl or vanadium radicals may be involved.In vivo subcutaneous injections of orthovanadate into mice containing MDAY-D2 tumours resulted in the inhibition of tumour growth by 85–100%. These data indicated that orthovanadate at concentrations greater than 5 uM has antineoplastic properties and may be useful as a chemotherapeutic agent.

Key words

orthovanadate antineoplastic cytotoxicity proliferation 

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Tony F. Cruz
    • 1
    • 2
  • Ariela Morgan
    • 1
    • 2
  • Weixian Min
    • 1
    • 2
  1. 1.Connective Tissue Research Group, Samuel Lunenfeld Research Institute, Department of PathologyMount Sinai HospitalTorontoCanada
  2. 2.University of TorontoTorontoCanada

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