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Fine structure, mechanism of heart function and haemodynamics in the prosobranch gastropod molluscLittorina littorea (L.)

Summary

The heart, main blood vessels, and associated structures ofLittorina littorea were examined by scanning and transmission electron microscopy. The auricle is subdivided into two compartments, one receiving blood from the gill and opening to the nephridial gland vein, the other connecting with the latter anteriorly and the ventricle posteriorly.

Video recordings were made of the beating heart in vivo and revealed that the auricle expelled blood not only to the ventricle, but also the nephridial gland vein at systole and provided further evidence of tidal flow of blood in the vein. There is clear indication that the constant volume mechanism of auricular re-filling is not strictly true inLittorina.

Blood pressure in the heart and major vessels was measured using a servo-nulling micropressure system. The rate of formation of urine (derived by filtration of blood through the auricular wall) was measured using [51Cr] EDTA as a blood marker.

Basal blood pressure was slightly above ambient (0.7 cm H2O). Peak systolic pressure in the ventricle (3.8 cm H2O) was synchronised with a subambient trough in pericardial pressure (−1.0 cm H2O); these pressure pulses were out of phase with that of the auricle (2.3 cm H2O) at systole. The observations are consistent in broad terms with a constant volume mechanism, but this does not take into account urine formation or filling of the nephridial gland vein.

A filtration pressure of 1.5 cm H2O has been demonstrated across the auricular wall throughout the cardiac cycle. Colloidal back pressure appears to be negligible. The mean rate of urine formation is 0.26 μl g−1 min−1.

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References

  1. Aardt WJ van (1968) Quantitative aspects of the water balance inLymnaea stagnalis (L.). Neth J Zool 18:253–312

  2. Andrews EB (1976) The fine structure of the heart of some prosobranch and pulmonate gastropods in relation to filtration. J Molluscan Stud 42:199–216

  3. Andrews EB (1979) Fine structure in relation to function in the excretory system of two species ofViviparus. J Molluscan Stud 45:186–206

  4. Andrews EB (1981) Osmoregulation and excretion in prosobranch gastropods, part 2. Structure in relation to function. J Molluscan Stud 47:248–289

  5. Andrews EB (1985) Structure and function in the excretory system of archaeogastropods and their significance in the evolution of gastropods. Phil Trans R Soc Lond B 310:383–406

  6. Andrews EB (1988) Excretory systems of molluscs. In: Trueman ER, Clarke M (eds) The Mollusca, vol 11. Form and function. Academic Press, Orlando, pp 381–448

  7. Bourne GB, Redmond JR (1977) Hemodynamics in the Pink Abalone,Haliotis corrugata (Mollusca, Gastropoda). 1. Pressure relations and pressure gradients in intact animals. J Exp Zool 200:9–16

  8. Civil GW, Thompson TE (1972) Experiments with the isolated heart of the gastropodHelix pomatia in an artificial pericardium. J Exp Biol 56:239–247

  9. Dale B (1974) The eco-physiological significance of the circulatory mechanics ofLymnaea stagnalis L. Comp Biochem Physiol 47A:1105–1113

  10. Delhaye W (1974) Histophysiologie comparée du rein chez Mésogastéropodes Archaeotaenioglossa et Littorinoidea (Mollusca-Prosobranchia). Arch Biol 85:461–507

  11. Depledge MH, Phillips DJH (1986) Circulation, respiration and fluid dynamics in the gastropod mollusc,Hemifusus tuba (Gmelin). J Exp Mar Biol Ecol 95:1–13

  12. Elder HY (1973) Distribution and functions of elastic fibers in the invertebrates. Biol Bull 144:43–63

  13. Elder HY, Owen G (1967) Occurrence of ‘elastic’ fibres in the invertebrates. J Zool Lond 152:1–8

  14. Florey E, Cahill MA (1977) Hemodynamics in lamellibranch molluscs: confirmation of constant-volume mechanism of auricular and ventricular filling. Remarks on the heart as site of filtration. Comp Biochem Physiol 57A:47–52

  15. Fretter V (1964) Observations on the anatomy ofMikadotrochus amabilis Bayer. Bull Mar Sci Gulf Caribb 14:172–184

  16. Fretter V (1966) Biological investigations of the deep sea. 16. Observations on the anatomy ofPerotrochus. Bull Mar Sci 16:603–614

  17. Fretter V (1982) An external vascular opening inLittorina spp. J Molluscan Stud 48:105

  18. Fretter V, Graham A (1962) British prosobranch molluscs. Ray Society, London

  19. Ghiretti F, Ghiretti-Magaldi A (1972) Respiratory proteins in molluscs. In: Florkin M, Scheer BT (eds) Chemical zoology, vol VII. Academic Press, London, pp 201–217

  20. Hevert F (1984) Urine formation in the lamellibranchs: evidence for ultrafiltration and quantitative description. J Exp Biol 111:1–12

  21. Jennings KH (1984) The organization, fine structure and function of the excretory system of the estuarine bivalve,Scrobicularia plana (da Costa) and the freshwater bivalveAnodonta cygnea (Linné) and other selected species. PhD thesis, University of London

  22. Jones HD (1970) Hydrostatic pressures within the heart and pericardium ofPatella vulgata L. Comp Biochem Physiol 34:201–272

  23. Jones HD (1971) Circulatory pressures inHelix pomatia L. Comp Biochem Physiol 39A:289–295

  24. Jones HD (1983) The circulatory systems of gastropods and bivalves. In: Wilbur KM, Saleuddin ASM (eds) The Mollusca, vol 5. Physiology 2. Academic Press, New York, pp 189–238

  25. Jones HD, Kamel EG (1984) The effect ofCryptocotyle lingua infection on the blood volume ofLittorina littorea. Comp Biochem Physiol 79A:493–494

  26. Kamel EG (1979) The physiological effects of platyhelminth parasites onLittorina littorea (L.). PhD thesis, University of Manchester

  27. Krijgsman BJ, Divaris GA (1955) Contractile and pacemaker mechanisms of the heart of molluscs. Biol Rev 30:1–39

  28. Little C (1965) The formation of urine by the prosobranch gastropod molluscViviparus viviparus Linn. J Exp Biol 43:39–54

  29. Little C (1967) Ionic regulation in the queen conch,Strombus gigas (Gastropoda, Prosobranchia). J Exp Biol 46:459–474

  30. Lowry DH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265

  31. Mangum CP, Johansen K (1975) The colloid osmotic pressures of invertebrate body fluids. J Exp Biol 63:661–671

  32. Martoja M, Tue BT, Elkaim B (1980) Bioaccumulation du cuivre chezLittorina littorea (L.) (Gastéropode prosobranche): signification physiologique et écologique. J Exp Mar Biol Ecol 43:215–270

  33. Potts WTW (1968) Aspects of excretion in the Mollusca. Symp Zool Soc London 22:187–192

  34. Potts WTW (1975) Excretion in gastropods. In: Wessing A (ed) Excretion. Fortschr Zool 23: 76–88

  35. Ramsay JA (1952) A physiological approach to the lower animals. University Press, Cambridge

  36. Riegel JA (1986a) Measurement of colloidal osmotic pressure of microlitre and sub-microlitre volumes. J Exp Biol 122:433–437

  37. Riegel JA (1986b) Hydrostatic pressures in glomeruli and renal vasculature of the hagfish,Eptatretus stouti. J Exp Biol 123:359–371

  38. Rolle G (1907–1908) Die Renoperikardialverbindung bei den einheimischen Nacktschnecken und anderen Pulmonaten. Jena Z Naturwiss 43:373–417

  39. Smith PJS (1987) Cardiac output in the Mollusca; scope and regulation. Experientia 43:956–965

  40. Sommerville BA (1973) The circulatory physiology ofHelix pomatia. III. The hydrostatic pressure changes in the circulatory system of livingHelix. J Exp Biol 59:291–303

  41. Taylor PM, Andrews EB (1987) Glucose resorption by the prosobranch gastropodLittorina littorea (L.). J Exp Mar Biol Ecol 108:99–111

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Andrews, E.B., Taylor, P.M. Fine structure, mechanism of heart function and haemodynamics in the prosobranch gastropod molluscLittorina littorea (L.). J Comp Physiol B 158, 247–262 (1988). https://doi.org/10.1007/BF01075839

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Keywords

  • Pressure Pulse
  • Cardiac Cycle
  • Systolic Pressure
  • Heart Function
  • Broad Term