The American Journal of Digestive Diseases

, Volume 23, Issue 10, pp 929–935

Changes in plasma motilin concentration and gastrointestinal contractile activity in conscious dogs

  • Zen Itoh
  • Shinjin Takeuchi
  • Isamu Aizawa
  • Kohzaburo Mori
  • Tomohiko Taminato
  • Yutaka Seino
  • Hiroo Imura
  • Noboru Yanaihara
Original Articles

Abstract

Simultaneous measurement of plasma motilin concentration and gastrointestinal contractile activity was made in 12 healthy dogs. Plasma motilin concentration was measured by radioimmunoassay and gastrointestinal contractile activity was monitored by means of chronically implanted force transducers. During the interdigestive state, it was found that the plasma motilin concentration increased in complete accordance with the cyclical interdigestive contractions of the stomach. Furthermore, plasma motilin concentration was lowered by the ingestion of food, and it remained low as long as the gastric motor activity was in the digestive pattern. Since, as reported previously, the interdigestive contractions can be induced by the exogenous administration of motilin, we concluded that (1) motilin is released at constant intervals during the interdigestive state, and this release is suppressed by the ingestion of food; (2) motilin induces the interdigestive contractions of the stomach and duodenum; and (3) motilin is an interdigestive hormone and is the only known polypeptide hormone of the gut whose release is not induced by a meal.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Itoh Z, Aizawa I, Takeuchi S, Couch EF: Hunger contractions and motilin. Proceedings of 5th International Symposium on Gastrointestinal Motility. G Vantrappen, ed. Herentals, Belgium, Typoff-Press, 1975; pp 48–55Google Scholar
  2. 2.
    Itoh Z, Honda R, Hiwatashi K, Takeuchi S, Aizawa I, Takayanagi R, Couch EF: Motilin-induced mechanical activity in the canine alimentary tract. Scand J Gastroenterol 11 (Suppl 39):93–110, 1976Google Scholar
  3. 3.
    Itoh Z, Takeuchi S, Aizawa I, Takayanagi R: Effect of synthetic motilin on gastric motor activity in conscious dogs. Am J Dig Dis 22:813–819, 1977Google Scholar
  4. 4.
    Mori K, Seino Y, Taminato A, Goto Y, Inoue Y, Kadowaki S, Matsukura S, Imura H: Radioimmunoassay for motilin. Presented at the 34th Annual Meeting of Japan Endocrinological Society at Okayama in May, 1977Google Scholar
  5. 5.
    Mori K, Seino Y, Taminato Y, Ikeda M, Matsukura S, Imura H. Yanaihara N: Secretory pattern of plasma motilin. Presented at the 43rd Annual Meeting of the Japanese Society of Gastroenterology in Tokyo in April, 1977Google Scholar
  6. 6.
    Shimizu F, Imagawa K, Mihara S, Yanaihara N: Synthesis of porcine motilin by fragment condensation using three protected peptide fragments. Bull Chem Soc Jpn 49:3594–3596, 1976Google Scholar
  7. 7.
    Itoh Z, Carlton N, Lucien HW, Schally AV: Long-term tubing implantation into the external jugular vein for injection or infusion in the dog. Surgery 66:768–770, 1969Google Scholar
  8. 8.
    Bass P, Wiley JN: Contractile force transducer for recording muscle activity in unanesthetized animals. J Appl Physiol 32:567–570, 1972Google Scholar
  9. 9.
    Itoh Z, Honda R, Takeuchi S, Aizawa I, Takayanagi R: An extraluminal force transducer for recording contractile activity of the gastrointestinal smooth muscle in the conscious dog: Its construction and implantation. Gastroenterol Jpn 12:275–283, 1977Google Scholar
  10. 10.
    Itoh Z, Aizawa I, Takeuchi S, Takayanagi R: Diurnal changes in gastric motor activity in conscious dogs. Am J Dig Dis 22:117–124, 1977Google Scholar
  11. 11.
    Yanaihara C, Sato H, Yanaihara N, Forssmann WG, Helmstaedter V: Motilin-, substance P-and somatostatin-like immunoreactivities in the extracts from dog, tupia and monkey brains and GI tracts. Abstract of International Symposium of Gastrointestinal Hormones and Pathology of the Digestive System. Speranza, Basso, Lezoche (eds). June 12–15, 1977, Rome, p 134Google Scholar
  12. 12.
    Hunter WM, Greenwood FC: Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 194:495–496, 1962Google Scholar
  13. 13.
    Albano JDM, Ekins RP, Turner RC: A sensitive, precise radioimmunoassay of serum insulin relying on charcoal separation of bound and free hormone moieties. Acta Endocrinol 70:487–509, 1972Google Scholar
  14. 14.
    Gerwirtz G, Yallow R: Ectopic ACTH production in carcinoma of the lung. J Clin Invest 53:1022–1030, 1974Google Scholar
  15. 15.
    Dryburgh JR, Brown JC: Radioimmunoassay for motilin. Gastroenterology 68:1169–1176, 1975Google Scholar
  16. 16.
    Ruppin H, Domschke S, Domschke W, Wünsch E, Demling L. Effects of 13-nie-motilin in man—inhibition of gastric evacuation and stimulation of pepsin secretion. Scand J Gastroenterol 10:199–202, 1975Google Scholar
  17. 17.
    Mitznegg P, Bloom SR, Domschke S, Domschke W, Wünsch E, Demling L. Release of motilin after duodenal acidification. Lancet 1:888–889, 1976Google Scholar
  18. 18.
    Mitznegg P, Bloom SR, Christofides N, Besterman H, Domschke W, Domschke S, Wünsch E, Demling L: Release of motilin in man. Scand J Gastroenterol 11 (Suppl 39):53–56, 1976Google Scholar
  19. 19.
    Wingate DL, Ruppin H, Thompson HH, Green WER, Domschke W, Wünsch E, Demling L, Rithie HD: 13-Norleucine motilin versus pentagastrin: Contrasting and competitive effects on gastrointestinal myoelectrical activity in the conscious dog. Acta Hepato-Gastroenterol 22:409–410, 1975Google Scholar
  20. 20.
    Wingate DL, Ruppin H, Green WER, Thompson HH: Motilin-induced electrical activity in the canine gastrointestinal tract. Scand J Gastroenterol 11 (Suppl 39):111–118, 1976Google Scholar
  21. 21.
    Jennewein HM, Bauer R, Hummelt H, Lepsin G, Siewert R: Motilin effects on gastrointestinal motility and lower esophageal sphincter (LES) pressure in dogs. Scand J Gastroenterol 11 (Suppl 39):63–65, 1976Google Scholar
  22. 22.
    Lee KY, Chey WY, Tai HH, Wagner D, Yajima H: Cyclic changes in plasma motilin levels and interdigestive myoelectric activity of canine antrum and duodenum. Gastroenterology 72:A-139/1162, 1977 (abstract).Google Scholar
  23. 23.
    Chey WY, Lee KY, Tai HH, Wagner D, Yajima H: The important role of vagus in the release of motilin and interdigestive myoelectric activity of the duodenum. Abstract of International Symposium on Gastrointestinal Hormones and Pathology of the Digestive System, Speranza, Basso, Lezoche (eds.) June 12–15, 1977, Rome, p 129Google Scholar
  24. 24.
    Bloom SR, Mitznegg P, Bryant MG: Measurement of human plasma motilin. Scand J Gastroenterol 11 (Suppl 39):47–52, 1976Google Scholar

Copyright information

© Digestive Disease Systems, Inc. 1978

Authors and Affiliations

  • Zen Itoh
    • 1
    • 2
    • 3
  • Shinjin Takeuchi
    • 1
    • 2
    • 3
  • Isamu Aizawa
    • 1
    • 2
    • 3
  • Kohzaburo Mori
    • 1
    • 2
    • 3
  • Tomohiko Taminato
    • 1
    • 2
    • 3
  • Yutaka Seino
    • 1
    • 2
    • 3
  • Hiroo Imura
    • 1
    • 2
    • 3
  • Noboru Yanaihara
    • 1
    • 2
    • 3
  1. 1.G.I. Laboratories, Department of SurgeryGunma University School of MedicineMaebashiJapan
  2. 2.Third Division, Department of Internal MedicineKobe University School of MedicineKobeJapan
  3. 3.Laboratory of Bio-organic ChemistryShizuoka College of Pharmaceutical ScienceShizuokaJapan

Personalised recommendations