The American Journal of Digestive Diseases

, Volume 20, Issue 5, pp 454–459

Immunoglobulin characterization of human pancreatic fluid

  • George W. Brasher
  • Walter P. Dyck
  • Frank F. Hall
  • A. Michael Spiekerman
Original Articles

Abstract

Human pancreatic fluid obtained from 2 subjects, each with a traumatic pancreatic fistula, contained detectable levels of IgG, IgA, IgM, IgD, and IgE. Although the mean IgG/IgA ratio for 10 random specimens was 1.63, the relative concentration was estimated to be less than unity when extreme values were eliminated. The molecular weight of IgA in pancreatic fluid was found to be comparable to that of the IgA molecule in serum. The absence of secretory component in pancreatic IgA provides further evidence that pancreatic IgA and serum IgA are similar. Serial determinations of the immunoglobulins stored at 4° C showed a progressive decrease of all immunoglobulins, the order of stability being IgG≈IgA>IgM≈IgD. The demonstrated proteolytic activity in the specimens could account for the immunoglobulin decay and for the variable detection of IgM and IgD in pancreatic-fluid specimens.

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Copyright information

© Digestive Disease Systems, Inc. 1975

Authors and Affiliations

  • George W. Brasher
    • 1
    • 2
  • Walter P. Dyck
    • 1
    • 2
  • Frank F. Hall
    • 1
    • 2
  • A. Michael Spiekerman
    • 1
    • 2
  1. 1.Department of Allergy and Immunology, Immunology Research Laboratory, Gastrointestinal Physiology and Research LaboratoriesScott and White ClinicTemple
  2. 2.Department of Clinical Pathology Section of BiochemistryScott and White ClinicTemple

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