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The detection of aberrant DNA synthesis in a member of a high-risk cancer family

Abstract

Relatives of patients with multiple polyposis are among those at high risk for development of neoplasms in the colon. Examination of 4 siblings, 3 men and 1 woman, of a patient with multiple polyposis was conducted for the possible presence of colonic polyps. All patients were over 40 years of age and received barium enemas for the radiological detection of excrescences. Proctoscopic examinations were also carried out during which time a biopsy and colonic wash were obtained. Polyps were absent on films as well as on endoscopy, and colonic cytologies of all 4 subjects were within normal limits. However, isotopic incorporation studies revealed the presence of an abnormal labeling pattern in some crypts of the biopsy incubated with TdR3H of 1 family member. Along with normal crypts with label in the lower two-thirds of the colonic crypts, some were seen to have cells labeled at the surface, a proliferative lesion thought to precede the appearance of a polyp. Among the surface cells removed by the colonic wash, some were found to be isotopically labeled, that is, engaged in DNA synthesis. Thus, a defect in the regulation of colonic epithelial cell replication was found, suggesting the need for close surveillance in the interest of early colon cancer detection.

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References

  1. 1.

    Deschner EE, Long FC, Katz S: Autoradiographic method for an expanded assessment of colonic cytology. Acta Cytol 17:435–438, 1973

  2. 2.

    Deschner EE, Lipkin M, Solomon C: Study of human rectal epithelial cellsin vitro. II. H3thymidine incorporation into polyps and adjacent mucosa. J Natl Cancer Inst 36:849–857, 1966

  3. 3.

    Bleiberg H, Maniguet P, Galand P: Cell renewal in familial polyposis: Comparison between polyps and adjacent healthy mucosa. Gastroenterology 63:240–245, 1972

  4. 4.

    Deschner EE, Lipkin M: Proliferative patterns in colonic mucosa in familial polyposis. Cancer, In press, 1975

  5. 5.

    Enquist IF: The incidence and significance of polyps of the colon and rectum. Surgery 42: 681–688, 1957

  6. 6.

    Corman ML, Veidenheimer MC, Coller JA: Barium-enema study findings in asymptomatic patients with rectal polyps. Dis Colon Rectum 17:325–330, 1974

  7. 7.

    Deschner EE, Lewis CM, Lipkin M:In vitro study of human rectal epithelial cells. I. Atypical zone of H3thymidine incorporation in mucosa of multiple polyposis. J Clin Invest 42:1922–1928, 1963

  8. 8.

    Deschner EE, Lipkin M: Study of human rectal epithelial cellsin vitro. III. RNA, protein and DNA synthesis in polyps and adjacent mucosa. J Natl Cancer Inst 44:175–185, 1970

  9. 9.

    Deschner EE: Autoradiographic studies of DNA, RNA and protein synthesis in normal and diseased colonic mucosa. Carcinoma of the Colon and Antecedent Epithelium. W Burdette (ed). Springfield, Illinois, Charles C Thomas Co, 1970, pp 222–229

  10. 10.

    Winawer SJ, Fleisher M, Melamed M, Sherlock P, Deschner E, Schwartz M: Cytological, immunological (CEA), and isotopic labeling studies based on gastrointestinal endoscopic lavage. Gastroenterology 66:800, 1974 (Abstract)

  11. 11.

    Posner G, Melamed M, Deschner EE, Boyle C, Winawer SJ: Sequential cytologic and autoradiographic studies in developing colon cancer in a patient with familial polyposis. Gastroenterology 64:785, 1973 (Abstract)

  12. 12.

    Deschner EE: Early detection of colonic polyps. Am J Clin Path 62:140–141, 1974 (Abstract)

  13. 13.

    Katz S, Sherlock P, Winawer SJ: Rectocolonic exfoliative cytology: A new approach. Am J Dig Dis 12:1109–1116, 1972

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Author information

Correspondence to Dr. Eleanor E. Deschner PhD.

Additional information

This work was supported by NCI Grant Ca08748 and NCI Contract 7-2041.

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Deschner, E.E., Long, F.C. & Katz, S. The detection of aberrant DNA synthesis in a member of a high-risk cancer family. Digest Dis Sci 20, 418–424 (1975). https://doi.org/10.1007/BF01070785

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Keywords

  • Colonic Polyp
  • Colonic Epithelial Cell
  • Label Pattern
  • Colonic Crypt
  • Early Colon