Sixty-six rats of seven inbred strains were observed in an open field (OF) and in a light-dark preference box (LDB). Each set of apparatus provides measures of locomotor activity and of the time spent in the “most protected” area (the corners in the OF, the dark compartment in the LDB). These measures are proposed to be indices of emotionality. In the present study, the reliability and validity of the OF and LDB measures were determined by genetic correlations. Genetic correlations (rGEN) within and between tests were used as they provide a more coherent picture of reliability and validity of the measures than productmoment correlations (rPM), since rGEN's are appropriate estimates of “true” individual scores. It appeared that the activity measure in the OF and the occupancies of the most protected parts of OF and LDB were valid indices of emotionality, while the activity measure in the LDB was not. We recommend that the occupancy measures in the OF and the LDB should be used in addition to the commonly used activity measure in the OF to assess emotionality. The results indicate that genetic correlations provide a useful tool to validate measures in animal research. The only experimental manipulation in this approach consists of using the variation between inbred strains.
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Aulich, D. (1976). Escape versus exploratory activity: An interpretation of rats' behaviour in the open field and a light-dark preference test.Behav. Process. 1:153–164.
Aulich, D., and Spielhofen, J. (1977). Effects of situational complexity and repeated testing on rats' behaviour in a light-dark preference situation.Z. Tierpsychol. 44:148–153.
Blizard, D. A., and Bailey, D. W. (1979). Genetic correlation between open-field activity and defecation: Analysis with the CxB recombinant-inbred strains.Behav. Genet. 9:349–357.
Broadhurst, P. L. (1957). Determinants of emotionality in the rat. I. Situational factors.Br. J. Psychol 48:1–12.
Broadhurst, P. L. (1960). Experiments in psychogenetics. In Eysenck, H. J. (Ed.),Experiments in Personality, Vol. 1, Routledge & Kegan Paul, London, pp. 1–102.
Campbell, D. T., and Fiske, D. W. (1959). Convergent and discriminant validation by the multitraitmultimethod matrix.Psychol Bull. 56:81–105
Dalrymple-Alford, J. C., and Benton, D. (1981). Activity differences of individually and grouphoused male and female rats.Anim. Learn. Behav. 9:50–55.
Durcan, M. J., Wraight, K. B., and Fulker, D. W. (1984). The current status of two sublines of the Roman High and Low Avoidance strains.Behav. Genet. 14:559–569.
Goma, M., and Tobeña, A. (1978). Reliability of various measures obtained in open-field test.Psychol. Rep. 43:1123–1128.
Hall, C. S. (1934). Emotional behavior in the rat. I. Defecation and urination as measures of individual differences in emotionality.J. Comp. Physiol. Psychol. 18:385–403.
Hall, C. S. (1936). Emotional behavior in the rat. III. The relationship between emotionality and ambulatory activity.J. Comp. Physiol. Psychol. 22:345–352.
Hegmann, J. P., and Possidente, B. (1981). Estimating genetic correlations from inbred strains.Behav. Genet. 11:103–114.
Hirsjärvi, P. A., and Junnila, M. A. (1986). Effects of light and noise test stimuli on the open-field behavior of Wistar rats.Scand. J. Psychol. 27:311–319.
Morrison, B. J., and Thatcher, K. (1969). Overpopulation effects on social reduction of emotionality in the albino rat.J. Comp. Physiol. Psychol. 69:658–662.
Ossenkopp, K.-P., and Mazmanian, D. S. (1985). The principle of aggregation in psychobiological correlational research: An example from the open-field test.Anim. Learn. Behav. 13:339–344.
Runkel, P. J., and McGrath, J. E. (1972).Research on Human Behavior, Holt, Rinehart and Winston, New York.
Tachibana, T. (1982). Open-field test for rats: Correlational analysis.Psychol. Rep. 50:899–910
Valle, F. P. (1970). Effects of strain, sex, and illumination on open-field behavior of rats.Am. J. Psychol. 83:103–111.
Valle, F. P. (1971). Rats' performance on repeated tests in the open field as a function of age.Psychon. Sci. 23:333–335.
van der Staay, F. J., Raaijmakers, W., and Kerbusch, S. (1983). Rapid acquisition of two-way active avoidance in inbred Roman Low Avoidance rats.Behav. Genet. 13:435–440.
van Luijtelaar, E. L. J. M., and Coenen, A. M. L. (1982). Differential behavioural effects of two instrumental paradoxical sleep deprivation techniques in rats.Biol. Psychol. 15:85–93.
Walsh, R. N., and Cummins, R. A. (1976). The open-field test: A critical review.Psychol. Bull. 83:482–504.
Welker, W. I. (1959). Escape, exploratory, and food-seeking responses of rats in a novel environment.J. Comp. Physiol. Psychol. 52:106–111.
Whimbey, A. E., and Denenberg, V. H. (1967). Two independent behavioral dimensions in open-field performance.J. Comp. Physiol. Psychol. 63:500–504.
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Josef van der Staay, J., Kerbusch, S. & Raaijmakers, W. Genetic correlations in validating emotionality. Behav Genet 20, 51–62 (1990). https://doi.org/10.1007/BF01070740
- genetic correlations
- inbred rat strains