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Changes in activity of avian serum esterases following exposure to organophosphorus insecticides


Starlings (Sturnus vulgaris) were dosed with corn oil, demeton-S-methyl (S-[2-(ethyl thio)ethyl]O,O dimethyl phosphorothioate) or triazophos (O,O-diethyl-O-(1-phenyl-1H-1,2,4-triazol-3-yl)phosphorothioate). Brain and serum esterase activities were measured up to 24 h after dosing. A dose response relationship was observed in serum cholinesterase and carboxylesterase activities following dosing with both pesticides. An increase in serum carboxylesterase activity was observed following exposure to a low dose of demeton-S-methyl, whereas inhibition was observed at higher doses. These results highlighted the need for the development of a method for monitoring exposure which takes into account both release and inhibition of the enzyme.

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  1. Aldridge WN (1953) Serum esterases. Biochem J 53:110–124

  2. Aldridge WN, Reiner E (1972) Enzyme inhibitors as substrates. Interactions of esterases with esters of organophosphorus and carbamic acids. Neuberger A, Tatum EL (eds) Frontiers of biology, Vol 26. North Holland Publishing Co., Amsterdam

  3. Bunyan PJ, Taylor A (1966) Esterase inhibition in pheasants poisoned by Thimet. J Agric Food Chem 14:132–137

  4. Bunyan PJ, Jennings DM, Taylor A (1968) Organophosphate poisoning: Some properties of avian esterases. J Agric Food Chem 16:326–331

  5. Bunyan PJ, Jennings DM, Taylor A (1969) Organophosphate poisoning: Chronic feeding of some common pesticides to pheasants and pigeons. J Agric Food Chem 17:1027–1032

  6. Devonshire AL (1977) The properties of a carboxylesterase from peach potato aphid and its role in conferring insecticide resistance. Biochem J 167:675–683

  7. Ellman GL, Courtney KD, Andreas Jr V, Featherstone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmac 7:88–95

  8. Fleming WJ, Bradbury SP (1981) Recovery of ChE activity in mallard ducklings administered organophosphorus pesticides. J Toxicol Environ Health 8:885–897

  9. Garcia-Rodriguez T, Ferrer M, Recio F, Castroviejo J (1987) Circadian rhythms of determined blood chemistry values in buzzards and eagle owls. Comp Biochem Physiol 88A:663–669

  10. Gomori G (1953) Human esterases. J Lab Clin Med 42:445–453

  11. Hemingway J, Rubio Y, Bobrowicz KE (1986) The use of ELISA demonstrates the absence ofCulex OP-resistance-associated esterases inAnopheles species. Pestic Biochem Physiol 25:327–335

  12. Ludke JL, Hill EF, Dieter MP (1975) ChE response and related mortality among birds fed ChE inhibitors. Arch Environ Contam Toxicol 3:1–21

  13. Mackness MI, Thompson HM, Hardy AR, Walker CH (1988) Distinction between ‘A’ esterases and arylesterases-implications for esterase classification. Biochem J 245:293–296

  14. Stedman E, Stedman E (1935) The relative cholinesterase activities of serum and corpuscles from the blood of certain species. Biochem J 29:2107–2111

  15. Thompson HM, Walker CH, Hardy AR (1988) Avian esterases as indicators of exposure to insecticides—the factor of diurnal variation. Bull Environ Contam Toxicol 41:4–11

  16. Thompson HM, Tarrant KA, Hart ADM (1991) Exposure of starlings, house sparrows and skylarks to pesticides. In: GreigSmith PW, Frampton GK, Hardy AR (eds) The Boxworth Project: Pesticides, cereal farming and the environment. HMSO, London. In press

  17. Vandekar M (1980) Minimizing occupational exposure to pesticides -ChE determination and OP poisoning. Residue Reviews 75:67–80

  18. Wallace KM, Herzberg U (1988) Reactivation and aging of phosphorylated brain acetylcholinesterase from fish and rodents. Toxicol Appl Pharmacol 92:307–314

  19. Westlake GE, Blunden CA, Brown PM, Bunyan PJ, Martin AD, Sayers PE, Stanley PI, Tarrant KA (1980) Residues and enzyme changes in wood mice from the use of chlorfenvinphos and an organomercurial fungicide on winter wheat seed. Ecotoxicol Environ Safety 4:1–16

  20. Westlake GE, Bunyan PJ, Martin AD, Stanley PI, Steed LC (1981) Organophosphate poisoning: Effects of selected organophosphate pesticides on plasma enzymes and brain esterases of Japanese quail. J Agric Food Chem 29:772–778

  21. Zinkl JG, Henny CJ, Shea PJ (1979) Brain cholinesterase activities of passerine birds in a forest sprayed with cholinesterase inhibiting insecticides In: Animals as monitors of environmental pollutants. Proceedings of a symposium on pathology of environmental pollutants. University of Connecticut. National Academy of Science, Washington, DC. pp 356–365

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Thompson, H.M., Walker, C.H. & Hardy, A.R. Changes in activity of avian serum esterases following exposure to organophosphorus insecticides. Arch. Environ. Contam. Toxicol. 20, 514–518 (1991).

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  • Corn
  • Dose Response
  • Cholinesterase
  • Response Relationship
  • Thio