Journal of Neuro-Oncology

, Volume 21, Issue 3, pp 243–247 | Cite as

Cytokine gene expression on glioma cell lines and specimens

  • Ryuya Yamanaka
  • Ryuichi Tanaka
  • Takafumi Saitoh
  • Syougo Okoshi
Laboratory Investigation


Expression of the cytokine genes in human glioma cell lines and specimens was studied. Primers for 7 different human cytokine, IL-1Β, IL-6, IL-8, GM-CSF, TGF-Β1, TNF-α and IFN-γ were used to analyze messenger RNA transcripts by polymerase chain reaction (PCR). Messenger RNA encoding for IL-1Β, IL-6, IL-8, GM-CSF and TGF-Β1 was found to be expressed in some of glioma cell lines. And those showed a proliferative response to IL-1Β. IL-8 mRNA was found in 2 of 5 low grade gliomas, in 8 of 9 high grade gliomas. TGF-Β1 mRNA was found in all gliomas, in 1 of 2 normal brains. IL-1Β mRNA was only found in normal brains. TNF-α and IFN-γ were not found in glioma cell lines and specimens. IL-8 mRNA was apt to be found more frequently among high grade glioma specimens.

Key words

glioma IL-8 TGF-Β1 RT-PCR 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Ryuya Yamanaka
    • 1
  • Ryuichi Tanaka
    • 1
  • Takafumi Saitoh
    • 1
  • Syougo Okoshi
    • 2
  1. 1.Department of NeurosurgeryBrain Research InstituteJapan
  2. 2.Third Internal MedicineNiigata UniversityNiigataJapan

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