Journal of Neuro-Oncology

, Volume 21, Issue 3, pp 243–247 | Cite as

Cytokine gene expression on glioma cell lines and specimens

  • Ryuya Yamanaka
  • Ryuichi Tanaka
  • Takafumi Saitoh
  • Syougo Okoshi
Laboratory Investigation

Abstract

Expression of the cytokine genes in human glioma cell lines and specimens was studied. Primers for 7 different human cytokine, IL-1Β, IL-6, IL-8, GM-CSF, TGF-Β1, TNF-α and IFN-γ were used to analyze messenger RNA transcripts by polymerase chain reaction (PCR). Messenger RNA encoding for IL-1Β, IL-6, IL-8, GM-CSF and TGF-Β1 was found to be expressed in some of glioma cell lines. And those showed a proliferative response to IL-1Β. IL-8 mRNA was found in 2 of 5 low grade gliomas, in 8 of 9 high grade gliomas. TGF-Β1 mRNA was found in all gliomas, in 1 of 2 normal brains. IL-1Β mRNA was only found in normal brains. TNF-α and IFN-γ were not found in glioma cell lines and specimens. IL-8 mRNA was apt to be found more frequently among high grade glioma specimens.

Key words

glioma IL-8 TGF-Β1 RT-PCR 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Fontana A, Hengartner H, de Tribolet N, Weber E: Glioblastoma cells release interleukin 1 and factors inhibiting interleukin 2 mediated effects. J Immunol 132: 1837–1844, 1984PubMedGoogle Scholar
  2. 2.
    Lichter T, Dohrmann GJ, Gurney ME: Cytokine gene expression by human gliomas. Neurosurgery 6: 788–793, 1990Google Scholar
  3. 3.
    Frei K, Bodmer S, Schwerde C, Fontana A: Astrocytes of the brain synthesize interleukin-3 like factors. J Immunol 135: 4044–4047, 1985PubMedGoogle Scholar
  4. 4.
    Yasukawa K, Hirano T, Watanabe Y, Muratani K, Matsuda T, Nakai S, Kishimoto T: Structure and expression of human B cell stimulatory factor-2(BSF-2/IL-6). EMBO J 6: 2939–2945, 1987PubMedGoogle Scholar
  5. 5.
    Larsson I, Landstrom LE, Larnor E, Lundgren E, Miorner H, Strannegard O: Interferon production in glia and glioma cell lines. Infect Immun 22: 786–789, 1978PubMedGoogle Scholar
  6. 6.
    Bodmer S, Siepl C, Fontana A: Immunoregulatory factors secreted by glioblastoma cells: glioblastoma derived T-cell suppressor factor/transforming growth factor-Β2. In: Goetzl EJ, Spector NH (eds) Neuroimmune Networks: Physiology and Diseases. New York, Alan R. Liss, Inc, 1989, pp 73–82Google Scholar
  7. 7.
    Takiguchi M, Ting JPY, Buessow SC, Boyer C, Gillespie Y, Frelinger JA: Response of glioma cells to γ-interferon. Eur J Immunol 15: 809–814, 1985PubMedGoogle Scholar
  8. 8.
    Kawano M, Hirano T, Matsuda T, Taga T, Horii Y, Iwato K, Asaoku H, Tang B, Tanabe O, Tanaka H, Kuramoto A, Kishimoto T: Autocrine generation and requirement of BSF-2/IL-6 for multiple myelomas. Nature 332: 83–85, 1988PubMedGoogle Scholar
  9. 9.
    Klein B, Zhang XG, Jourdan M, Cotent J, Houssiau F, Aarden L, Peichaczyk M, Bataille R: Paracrine rather than autocrine regulation of myeloma-cell growth and differentiation by interleukin-6. Blood 73: 517–526, 1989PubMedGoogle Scholar
  10. 10.
    Yamazaki K: Tumorigenicity of established human glioma cell lines in lasat and nude mice. Neuropathol 3: 29–38, 1982Google Scholar
  11. 11.
    Nishiyama A, Onda K, Washiyama K, Kumanishi T, Kuwano R, Sakimura K, Takahashi Y: Differential expression of glial fibrillary acidic protein in human glioma cell lines. Acta Neuropath 78: 9–15, 1989PubMedGoogle Scholar
  12. 12.
    Yamanaka R, Tanaka R, Yoshida S: Effects of irradiation on cytokine production in glioma cell lines. Neurol Med Chir (Tokyo) 33: 744–748, 1993Google Scholar
  13. 13.
    Chirgwin JM, Przybyia AE, McDonald RJ, Rutter WJ: Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294, 1979PubMedGoogle Scholar
  14. 14.
    Verlaan-de VM, Bogaard ME, Van dem Elst H, Van Boom JH, Van der Eb AJ, Bos JL: A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides. Gene 50: 313–320, 1986PubMedGoogle Scholar
  15. 15.
    Larsen CG, Anderson AO, Appella E, Oppenheim J, Matsushima K: The neutrophile-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science 243: 1464–1466, 1989PubMedGoogle Scholar
  16. 16.
    Van Damme J, Beeumen J, Opdenakker G, Billiau A: A novel, NH2-terminal sequence characterized human monokine possessing neutrophil chemotactic, skin reactive and granulocytosis-promoting activity. J Exp Med 167: 1364–1376, 1988PubMedGoogle Scholar
  17. 17.
    Yoshimura T, Matsushima K, Oppenheim J, Leonard E: Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: partial characterization and separation from interleukin 1 (IL1). J Immunol 139: 788–793, 1987PubMedGoogle Scholar
  18. 18.
    Koch AE, Polverini PJ, Kunkel SL: Interleukin-8 as a macrophage-derived mediator of antigiogenesis. Science 258: 1798, 1992PubMedGoogle Scholar
  19. 19.
    Kuppner MC, Hamou MF, Sawamura Y, Bodmer S, de Tribolet N: Inhibition of lymphocyte function by glioblastomaderived transforming growth factor Β2. J Neurosurg 71: 211–217, 1989PubMedGoogle Scholar
  20. 20.
    Naganuma H, Sasaki A, Satoh E, Sakihama T, Tasaka K, Nukui H: Improved bioassay of the detection of transforming growth factor-Β1 and Β2 in malignant gliomas. Neurol Med Chir (Tokyo) 34: 143–149, 1994Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Ryuya Yamanaka
    • 1
  • Ryuichi Tanaka
    • 1
  • Takafumi Saitoh
    • 1
  • Syougo Okoshi
    • 2
  1. 1.Department of NeurosurgeryBrain Research InstituteJapan
  2. 2.Third Internal MedicineNiigata UniversityNiigataJapan

Personalised recommendations