Neurophysiology

, Volume 9, Issue 4, pp 332–337 | Cite as

Effect of imidazole, guanidine, and theophylline on transmitter release in the frog neuromuscular synapse in relation to temperature and calcium ion concentration in the medium

  • I. M. Gotgil'f
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Abstract

The effects of imidazole, guanidine, and theophylline on spontaneous (frequency of miniature end-plate potentials) and evoked (quantum composition of end-plate potentials) transmitter release were compared in isolated sartorius muscles ofRana temporaria at different temperatures and during changes in the calcium concentration in the external solution. All three substances increased the quantum composition of the end-plate potentials and the frequency of the miniature end-plate potentials at 20°C and in 0.5 mM calcium. As regards their effect on the quantum composition the substances could be arranged in the following order: imidazole ≈ guanidine ≫ theophylline; as regards their effect on frequency: theophylline ≫ imidazole ≈ guanidine. Theophylline increased spontaneous release, whereas imidazole and guanidine increased evoked transmitter release more than the rest. Comparison of the effect of these substances at 20 and 7°C showed that only the action of theophylline on spontaneous release depends on temperature. The effect of imidazole and theophylline on frequency was independent of the calcium concentration in the medium. Differences in the mechanism of action of these compounds on spontaneous and evoked acetylcholine release are discussed.

Keywords

Imidazole Acetylcholine Theophylline Calcium Concentration Guanidine 
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© Plenum Publishing Corporation 1978

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  • I. M. Gotgil'f

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