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Effect of pentobarbital, chloralose, and urethane on inhibitory postsynaptic potentials of cortical neurons

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Abstract

The effect of pentobarbital, chloralose, and urethane on IPSPs arising in auditory cortical neurons in response to electrical stimulation of geniculocortical fibers was studied in experiments on cats immobilized with D-tubocurarine. Pentobarbital (60–80 mg/kg body weight, intraperitoneally) sharply reduced the number of neurons responding by spikes to geniculocortical stimulation. Only short-latency responses remained. The number of neurons responding with IPSPs was unchanged. Pentobarbital increased the duration of the IPSPs by 1.5–2 times and shortened their latent periods. Under the influence of chloralose (60 mg/kg, intraperitoneally) the number of responses of EPSP—spike—IPSP type was increased and the duration of the IPSPs also was increased by 3–4 times. The latent period of the primary IPSPs was shortened. Unlike pentobarbital and chloralose, urethane (1000 mg/kg, intravenously) reduced the duration of the IPSPs to 30 msec. About 2% of IPSPs recorded before anesthesia had a latent period of 1.0–1.5 msec. Under the influence of anesthesia the relative number of these IPSPs increased to 5.7%. It it postulated that they are monosynaptic. The mechanism of action of general anesthetics on the cortical inhibitory system is discussed.

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Literature cited

  1. 1.

    V. S. Arutyunov, V. I. Maloletnev, and S. P. Narikashvili, "Changes in the activity of single polysensory neurons of the cat association cortex under the influence of pentobarbital," Zh. Vyssh. Nerv. Deyat.,20, 1043 (1970).

  2. 2.

    É. É. Grangstrem, "Descending connections of the auditory cortex in the structure of synapses of the inferior colliculi and medial geniculate body," in: Morphology of the Pathways and Connections of the Central Nervous System [in Russian], Nauka, Moscow (1965), pp. 53–61.

  3. 3.

    V. Mountcastle, "Some functional properties of the somatic afferent system," in: Theory of Communication in Sensory Systems [Russian translation], Mir, Moscow (1964), pp. 185–213.

  4. 4.

    S. P. Narikashvili, V. S. Arutyunov, and É. Guma, "Characteristics of response activity of single units of the cat association cortex," Zh. Vyssh. Nerv. Deyat.,18, 865 (1968).

  5. 5.

    F. N. Serkov and E. Sh. Yanovskii, "Unit responses of the auditory cortex to stimulation of geniculocortical fibers," Neirofiziologiya,4, 227 (1972).

  6. 6.

    F. N. Serkov, E. Sh. Yanovskii, and A. N. Tal'nov, "Functional properties distinguishing individual auditory cortical neurons," Neirofiziologiya,5, 236 (1973).

  7. 7.

    F. N. Serkov, E. Sh. Yanovskii, and A. N. Tal'nov, "Characteristics of cortical inhibitory neurons," Neirofiziologiya,6, 119 (1974).

  8. 8.

    A. I. Shapovalov, Cellular Mechanisms of Synaptic Transmission [in Russian], Meditsina, Moscow (1966).

  9. 9.

    N. A. Shvyrkova, "Changes in the convergence of excitation on cortical neurons after administration of some general anesthetics and psychotropic drugs," Byull. Éksperim. Biol. i Med.,49, No. 5, 7 (1970).

  10. 10.

    V. E. Amassian, "Evoked cortical unit activity in somatic sensory areas," Electroenceph. Clin. Neurophysiol.,5, 415 (1953).

  11. 11.

    U. Balis and R. R. Monroe, "The pharmacology of chloralose," Psychopharmacology,6, 1 (1964).

  12. 12.

    J. E. Desmedt and K. Mechelse, "Corticofugal projection from temporal lobe in cat and their possible role in acoustic discrimination," J. Physiol. (London),147, 17P (1959).

  13. 13.

    R. Dubner and L. T. Rutledge, "Recording and analysis of converging input upon neurons in cat association cortex," J. Neurophysiol.,27, 620 (1964).

  14. 14.

    J. C. Eccles, The Inhibitory Pathways of the Central Nervous System, Liverpool University Press, (1969).

  15. 15.

    S. D. Erulcar, I. E. Rose, and P. W. Davis, "Single unit activity in the auditory cortex of the cat," Bull. Johns Hopkins Hosp.,99, 55 (1956).

  16. 16.

    Y. Katsuki, K. Murata, N. Suga, and T. Tanuka, "Electrical activity of cortex auditory neurons of unanesthetized and unrestrained cat," Proc. Japan. Acad.,35, 571 (1959).

  17. 17.

    J. Krnjević, M. Randić, and W. Straughan, "Pharmacology of cortical inhibition," J. Physiol. (London),184, 78 (1966).

  18. 18.

    W. D. Larson and M. A. Major, "The effect of hexobarbital on the recurrent IPSP in cat motoneurons," Brain Res.,21, 309 (1970).

  19. 19.

    C.-L. Li and H. H. Jasper, "Microelectrode studies of the electrical activity of the cerebral cortex of the cat," J. Physiol. (London),121, 117 (1953).

  20. 20.

    L. C. G. Massopust and M. Ordy, "Auditory organization of the inferior colliculi in the cat," Exp. Neurol.,6, 464 (1962).

  21. 21.

    V. B. Mountcastle and T. P. Powell, "Neuronal mechanisms subserving cutaneous sensibility, with special reference to the role of afferent inhibition in sensory perception and discrimination," Bull. Johns Hopkins Hosp.,105, 201 (1959).

  22. 22.

    C. D. Richards, "On the mechanisms of barbiturate anaesthesia," J. Physiol. (London),277, 749 (1972).

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Additional information

A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 6, No. 4, pp. 339–349, July–August, 1974.

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Serkov, F.N., Yanovskii, E.S. & Tal'nov, A.N. Effect of pentobarbital, chloralose, and urethane on inhibitory postsynaptic potentials of cortical neurons. Neurophysiology 6, 263–271 (1974). https://doi.org/10.1007/BF01063477

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Keywords

  • Body Weight
  • Electrical Stimulation
  • Latent Period
  • Cortical Neuron
  • Pentobarbital