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Effect of sodium-free solutions on ionic currents of snail giant neurons

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Abstract

The ionic currents of the snail giant neurons were investigated by the voltage clamp method. The effect of sodium-free solutions on the inward and outward currents was studied. It is shown that the current entering the cells is created mainly by sodium ions. When a preparation is immersed into a solution not containing sodium ions, most neurons (tentatively neurons of type "a") "lose" the inward currents. In other neurons (tentatively of type "b") this process lasts 40 min and more. A number of peculiarities of type "b" neurons were noted. The response of the excitable membrane to conditioning polarization was also investigated. The data obtained permit the conclusion that 85–90% of the sodium-transfer system is activated in the case of a voltage clamp from the level of the resting potential.

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Additional information

A. A. Bogomolets Institute of Physiology, Kiev. Translated from Neirofiziologiya, Vol. 2, No. 3, pp. 314–320, May–June, 1970.

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Kryshtal', O.A., Parkhomenko, N.T. Effect of sodium-free solutions on ionic currents of snail giant neurons. Neurophysiology 2, 238–242 (1970). https://doi.org/10.1007/BF01063366

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Keywords

  • Ionic Current
  • Outward Current
  • Voltage Clamp
  • Excitable Membrane
  • Clamp Method