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The effects of ochratoxin A on postimplantation rat embryos in culture

  • K. Mayura
  • John F. Edwards
  • Elizabeth A. Maull
  • Timothy D. Phillips
Article

Abstract

The mycotoxin, ochratoxin A (OA), is a potentin vivo teratogen. Studies were performed to determine thein vitro effects of OA on postimplantation rat embryos. Embryos were explanted from pregnant Sprague-Dawley rats on day 10 of gestation and were cultured (within the yolk sac) for 45 hr in gassed rat serum containing OA at concentrations between 0 and 300 μg/mL. Gross morphology, histopathology and protein and DNA content of embryos were evaluated. An OA concentration-dependent reduction in yolk sac diameter, crownrump length, somite number count, and protein and DNA content was observed. Ochratoxin A treatment also resulted in an increase in the incidence of defective embryos. Malformations included: growth retardation, hypoplasia of the telencephalon, poor flexion, stunted limb bud development, underdeveloped sensory primordia and decreased mandibular and maxillary size. Histological examination demonstrated extensive OA-induced necrosis of embryonal mesodermal structures and neuroectoderm. Thus, the rat embryo in culture is a sensitive indicator of OA toxicity and may be useful for predicting developmental hazards associated with this mycotoxin.

Keywords

Number Count Somite Number Mesodermal Structure Defective Embryo Developmental Hazard 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1988

Authors and Affiliations

  • K. Mayura
    • 1
  • John F. Edwards
    • 1
    • 2
  • Elizabeth A. Maull
    • 1
  • Timothy D. Phillips
    • 1
  1. 1.Department of Veterinary Public HealthCollege of Veterinary MedicineUSA
  2. 2.Department of Veterinary Pathology, College of Veterinary MedicineTexas A&M UniversityCollege StationUSA

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