Bioprocess Engineering

, Volume 9, Issue 6, pp 245–248 | Cite as

Purification of alkaline protease of Rhizopus oryzae by foam fractionation

  • Rintu Banerjee
  • Rajeev Agnihotri
  • B. C. Bhattacharyya


Studies on the cost effective purification method is very much essential for the industrially important enzyme like protease. As foam fractionation is an important technique in downstream processing of the biologicals, purification of the crude proteolytic enzyme produced by Rhizopus oryzae has been attempted using converging-diverging foam fractionator. The effects of different parameters studied on the purification efficiency by foam fractionation were pH of the broth, air flow rate, initial liquid height. The results obtained were found quite encouraging at pH 10 with 1 LPM of air flow rate at low initial liquid height.


Enzyme Waste Water Purification Foam Fractionation 
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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Rintu Banerjee
    • 1
  • Rajeev Agnihotri
    • 1
  • B. C. Bhattacharyya
    • 1
  1. 1.Biotechnology unit Department of Chemical EngineeringIndian Institute of Technology KharagpurKharagpurIndia

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