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Patterns of plasma levels of prednisolone after oral administration in man

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A competitive protein-binding method was found suitable for the estimation of plasma prednisolone levels in men in whom endogenous steroids were suppressed by dexamethasone premedication. This method is rapid and precise and allows measurements of prednisolone in small blood samples after usual therapeutic doses of the drug. Using this technique, two preparations of the medication were compared, a conventional form and an enteric-coated formulation; except for the delay due to the coating, they were completely comparable, since maximum levels reached, areas under the plasma concentration-time curves, and apparent disappearance half-lifes were almost identical.

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  1. 1.

    B. E. P. Murphy. Some studies on the protein-binding of steroids and their application to the routine micro and ultramicro measurement of various steroids in body fluids by competitive protein-binding.J. Clin. Endocrinol. Metab. 27: 973–990 (1967).

  2. 2.

    R. Leclercq, G. Copinschi, and J. R. M. Franckson. Le dosage par compétition du cortisol plasmatique: Modification de la méthode de Murphy.Rev. Fr. Etud. Clin. Biol. 14: 815–819 (1969).

  3. 3.

    P. De Moor, R. Deckx, and O. Steeno. Influence of various steroids on the specific binding of cortisol.J. Endocrinol. 27: 355–356 (1963).

  4. 4.

    F. H. Lorentz. Ein neuer Constitutionindex.Klin. Wschr. 8: 348–351 (1929).

  5. 5.

    F. Moncloa, F. G. Peron, and R. I. Dorfman. The fluorometric determination of corticosterone in rat adrenal tissue and plasma: Effect of administering ACTH subcutaneously.Endocrinology 65: 717–724 (1959).

  6. 6.

    G. W. Snedecor. Query 92.Biometrics 8: 85–86 (1952).

  7. 7.

    R. Leclercq, I. B. Taljedal, and S. Wold. Evaluation of radio-isotope data in steroid assays based on competitive protein binding.Clin. Chim. Acta 36: 257–259 (1972).

  8. 8.

    W. A. Colburn and R. H. Buller. Radioimmunoassay for prednisolone.Steroids 21: 833–846 (1973).

  9. 9.

    R. E. Peterson. Metabolism of adrenocorticosteroids in man.Ann. N.Y. Acad. Sci. 82: 846–853 (1959).

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Author information

Correspondence to Raoul Leclercq.

Additional information

This work was supported by the Fonds de la Recherche Scientifique Médicale and was partially realized under contract of the Ministère Belge de la Politique Scientifique, within the framework of the association Euratom-Université de Bruxelles-University of Pisa.

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Leclercq, R., Copinschi, G. Patterns of plasma levels of prednisolone after oral administration in man. Journal of Pharmacokinetics and Biopharmaceutics 2, 175–187 (1974). https://doi.org/10.1007/BF01061507

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Key words

  • plasma prednisolone
  • competitive protein binding
  • oral administration of prednisolone