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Neurophysiology

, Volume 21, Issue 2, pp 189–192 | Cite as

Restoration of voltage-dependent antibrain antibody-inhibited calcium current by cyclic adenosine monophosphate

  • E. I. Solntseva
Article
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Abstract

A dual-microelectrode voltage clamp technique was used for recording voltage-dependent calcium current (Ica) in unidentified neurons isolated fromHelix pomatia. Neither intracellular injection of cyclic adenosine monophosphate (cAMP; 10 nA, 5 min) nor intracellular application of dibutyril-cAMP (dcAMP; 1 mM, 10–20 min) induced a change in normal Ica or produce a reversible 10–20% reduction in amplitude. Adding S-100 protein fraction antibodies to the external medium led to the onset of calcium-dependent inactivation of Ica, bringing amplitude of Ica down to 15±12% of its initial level. Either cAMP or dcAMP then restored inhibited Ica to 50±11% of its original level. It was found that the effects of cAMP on Ica of intact neurons depend on level of cytoplasmic Ca2+.

Keywords

Calcium Adenosine Initial Level Protein Fraction External Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1989

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  • E. I. Solntseva

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