, Volume 21, Issue 2, pp 189–192 | Cite as

Restoration of voltage-dependent antibrain antibody-inhibited calcium current by cyclic adenosine monophosphate

  • E. I. Solntseva


A dual-microelectrode voltage clamp technique was used for recording voltage-dependent calcium current (Ica) in unidentified neurons isolated fromHelix pomatia. Neither intracellular injection of cyclic adenosine monophosphate (cAMP; 10 nA, 5 min) nor intracellular application of dibutyril-cAMP (dcAMP; 1 mM, 10–20 min) induced a change in normal Ica or produce a reversible 10–20% reduction in amplitude. Adding S-100 protein fraction antibodies to the external medium led to the onset of calcium-dependent inactivation of Ica, bringing amplitude of Ica down to 15±12% of its initial level. Either cAMP or dcAMP then restored inhibited Ica to 50±11% of its original level. It was found that the effects of cAMP on Ica of intact neurons depend on level of cytoplasmic Ca2+.


Calcium Adenosine Initial Level Protein Fraction External Medium 
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© Plenum Publishing Corporation 1989

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  • E. I. Solntseva

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