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Effects of polychlorinated biphenyls at low dose levels in rats

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Abstract

Clophen® A-50, a commercial polychlorinated biphenyl (PCB) mixture, was administered orally to rats for six weeks at the dose levels of 2, 10, 50, 150, and 250 mg/kg. An accumulation of PCBs was found in the liver, the kidney, and in the adipose tissue of all dosed animals. The highest levels were observed in the fatty tissue, which were 10 and 40 times higher than those found in the liver and kidney, respectively. After six weeks treatment with 2 mg/kg, histopathological alterations were evident in the liver, manifest as centrolobular necroses. At the dose level of 10 mg/kg, increases were found in the liver weight and in serum cholinesterase activity. The serum bilirubin concentration and serum protein content were elevated at 50 mg/kg. The activities of serum glutamic oxalacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) however, were only increased at doses higher than 50 mg/kg. The levels of the serum lipids cholesterol and triglycerides were elevated at the doses of 2 mg and 50 mg/kg respectively. The urinary excretion of δ-aminolevulinic acid (ALA) and porphobilinogen (PBG) was enhanced at the dose levels between 50 and 250 mg/kg, whereas the urinary porphyrin levels remained unchanged. The no-effect level of repeated oral PCB administration in rats was less than 2 mg/kg.

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References

  1. Allen, J. R., and D. A. Norback: Polychlorinated biphenyl and triphenyl induced mucosal hyperplasia in primates. Science179, 498 (1973).

  2. Allen, J. R., L. A. Carstens, and D. A. Barsotti: Residual effects of short-term, low-level exposure of nonhuman primates to polychlorinated biphenyls. Toxicol. Appl. Pharmacol.30, 440 (1974).

  3. Barsotti, D. A., R. J. Marlar, and J. R. Allen: Reproductive dysfunction in rhesus monkeys exposed to low levels of polychlorinated biphenyls (Aroclorr 1248). Food Cosmet. Toxicol.14, 99 (1976).

  4. Baumann, M.: Short term effects of Clophen® A-50 and of dichlorobiphenyl in rats. Arch. Toxicol. Suppl.2, 311 (1979).

  5. Benthe, H. F., and A. Schmoldt: Zur Persistenz von polychlorierten Biphenylen (PCB) an Ratten. Arch. Toxicol.30, 207 (1973).

  6. Braunberg, R. C., R. E. Dailey, E. A. Brouwer, L. Kasza, and A. M. Blaschka: Acute, subacute, and residual effects of polychlorinated biphenyl (PCB) in rats. I. Biologic half-life in adipose tissue. J. Toxicol. Environ. Health1, 683 (1976).

  7. Bruckner, J. V., K. L. Khanna, and H. H. Cornish: Biological responses of the rat to polychlorinated biphenyls. Toxicol. Appl. Pharmacol.24, 434 (1973).

  8. —: Polychlorinated biphenyl-induced alteration of biologic parameters in the rat. Toxicol. Appl. Pharmacol.28, 189 (1974).

  9. Doss, M., and A. Schmidt: Quantitative Bestimmung von δ-Aminolävulinsäure und Porphobilinogen im Urin mit Ionenaus-tauschchromatographie-Fertigsäulen. Z. Klin. Chem. Klin. Biochem.9, 99 (1971a).

  10. —: Rapid determination of urinary total porphyrins by ion exchange chromatography. Z. Klin. Chem. Klin. Biochem.9, 415 (1971b).

  11. Eggstein, M., and F. H. Kreutz: Eine neue Bestimmung der Neutralfette im Blutserum und Gewebe. I. Mitteilung: Prinzip, Durchführung und Besprechung der Methode. Klin. Wschr.44, 262 (1966).

  12. Ellman, G. L., K. D. Courtney, V. Andris Jr., and R. M. Featherstone: A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol.7, 88 (1961).

  13. Fawcett, J. K., and J. E. Scott: A rapid and precise method for the determination of urea. J. Clin. Pathol.13, 156 (1960).

  14. Fishbein, L.: Toxicity of chlorinated biphenyls. Ann. Rev. Pharmacol.14, 139 (1974).

  15. Goldstein, J. A., P. Hickman, and D. L. Jue: Experimental hepatic porphyria induced by polychlorinated biphenyls. Toxicol. Appl. Pharmacol.27, 437 (1974).

  16. Goldstein, J. A., P. Hickman, V. W. Burse, and H. Bergman: A comparative study of two polychlorinated biphenyl mixtures (Aroclors® 1242 and 1016) containing 42% chlorine on induction of hepatic porphyria and drug metabolizing enzymes. Toxicol. Appl. Pharmacol.32, 461 (1975).

  17. Hansell, M. M., and D. J. Ecobichon: Effects of chemically pure chlorobiphenyls on the morphology of rat liver. Toxicol. Appl. Pharmacol.28, 418 (1974).

  18. Holmes, D. C., J. H. Simmons, and J. O'G. Tatton: Chlorinated hydrocarbons in British wildlife. Nature216, 227 (1967).

  19. Jendrassik, L., and P. Grof: Vereinfachte photometrische Methoden zur Bestimmung des Blutbilirubins. Biochem. Z.297, 81 (1938).

  20. Karmen, A.: A note on the spectrophotometric assay of glutamicoxalacetic transaminase in human blood serum. J. Clin. Invest.34, 131 (1955).

  21. Kimbrough, R., J. Buckley, L. Fishbein, G. Flamm, L. Kasza, W. Marcus, S. Shibko, and R. Teske: Animal toxicology. Environ. Health Perspect.24, 173 (1978).

  22. Koller, L. D., and J. G. Zinkl: Pathology of polychlorinated biphenyls in rabbits. Amer. J. Pathol.70, 363 (1973).

  23. Litterst, C. L., T. M. Farber, A. M. Baker, and E. J. van Loon: Effect of polychlorinated biphenyls on hepatic microsomal enzymes in the rat. Toxicol. Appl. Pharmacol.23, 112 (1972).

  24. Mücke, W., G. Weigand, H. Greim, and H. Schulze: Schadstoffe in Lebensmitteln—Problemfall Organohalogenverbindungen in Muttermilch. Teil 1: Situation, Trendabschätzung und Höchstmengen. Z. für das gesamte Lebensmittelrecht8, 117 (1981).

  25. Nishizumi, M.: Light and electron microscope study of chlorobiphenyl poisoning. Arch. Environ. Health21, 620 (1970).

  26. Norback, D. H., and J. R. Allen: Chlorinated aromatic hydrocarbon induced modifications of the hepatic endoplasmic reticulum: concentric membrane arrays. Environ. Health Perspect.1, 137 (1972).

  27. Popper, H., E. Rubin, D. Gardiol, F. Schaffner, and F. Paronetto: Drug-induced liver disease. Arch. Intern. Med.115, 128 (1965).

  28. Risebrough, R. W., and B. de Lappe: Accumulation of polychlorinated biphenyls in ecosystems. Environ. Health Perspect.1, 39 (1972).

  29. Röschlau, P., E. Bernt, and W. Gruber: Enzymatische Bestimmung des Gesamt-Cholesterins im Serum. Z. Klin. Chem. Klin. Biochem.12, 226 (1974).

  30. Schäffer, E., H. Greim, and M. Baumann: Durch PCBs induzierte Leberveränderungen bei der Ratte. Berl. u. Münch. Tierärztl. Wschr. (in press).

  31. Schmoldt, A., H. F. Benthe, and W. Bürgener: Lipidveränderungen der Rattenleber nach Exposition mit Aerosolen poly-chlorierter Biphenyle (PCB). Int. Arch. Arbeitsmed.33, 183 (1974).

  32. Seelig, H. P., and H. Wüst: Die Kreatininbestimmung mit der Jaffé-Reaktion. Ärztl. Lab.15, 34 (1969).

  33. Sivalingan, P. M., T. Yoshida, and Y. Inada: The modes of inhibitory effects of PCBs on oxidative phosphorylation of mitochondria. Bull. Environ. Contam. Toxicol.10, 242 (1973).

  34. Sümmermann, W., H. Rohleder, and F. Korte: Polychlorierte Biphenyle (PCB) in Lebensmitteln. Z. Lebensm. Unters.-Forsch.166, 137 (1978).

  35. van Kampen, E. J., and W. G. Zijlstra: Standardization of hemo-globinometry. II. The hemiglobincyanide method. Clin. Chim. Acta6, 538 (1961).

  36. Vos, J. G., and J. H. Koeman: Comparative toxicologic study with polychlorinated biphenyls in chickens with special reference to porphyria, edema formation, liver necrosis, and tissue residues. Toxicol. Appl. Pharmacol.17, 656 (1970).

  37. Vos, J. G., and R. B. Beems: Dermal toxicity studies of technical polychlorinated biphenyls and fractions thereof in rabbits. Toxicol. Appl. Pharmacol.19, 617 (1971).

  38. Vos, J. G., and E. Notenboom-Ram: Comparative toxicity study of 2,4,5,2′,4′,5′-hexachlorobiphenyl and a polychlorinated biphenyl mixture in rabbits. Toxicol. Appl. Pharmacol.23, 563 (1972).

  39. Wassermann, M., D. Wassermann, S. Cucos, and H. J. Miller: World PCBs map: Storage and effects in man and his biologic environment in the 1970s. Annals N.Y. Acad. Sci.320, 69 (1979).

  40. Weichselbaum, T. E.: An accurate and rapid method for the determination of proteins in small amounts of blood serum and plasma. Amer. J. Clin. Pathol.16, 40 (1946).

  41. Wroblewski, F., and J. S. LaDue: Serum glutamic-pyruvic transaminase in cardiac and hepatic disease. Proc. Soc. Exp. Biol. Med.91, 569 (1956).

  42. Yakushiji, T., I. Watanabe, K. Kuwabara, S. Yoshida, K. Koyama, I. Hara, and N. Kunita: Long-term studies of the excretion of polychlorinated biphenyls (PCBs) through the mother's milk of an occupationally exposed worker. Arch. Environ. Contam. Toxicol.7, 493 (1978).

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Baumann, M., Deml, E., Schäffer, E. et al. Effects of polychlorinated biphenyls at low dose levels in rats. Arch. Environ. Contam. Toxicol. 12, 509–515 (1983). https://doi.org/10.1007/BF01056545

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Keywords

  • Porphyrin
  • PCBs
  • Dose Level
  • Porphobilinogen
  • Serum Glutamic Pyruvic Transaminase