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Developmental and methylmercury effects on brain protein synthesis

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Abstract

Sprague-Dawley rats (ages 1 to 21 days, alternate days) were chosen for study. Animals were injected with methylmercuric chloride (8 mg/kg IP); 12 hr later each animal was injected with uniformly labeled L-(14C)leucine, allowed 30 min for incorporation, and decapitated. Brains were analyzed for amounts of radioactivity incorporated into TCA precipitable protein. When compared to baseline curves for control animals, curves of treated animals were compressed overall and had age-dependent increases and decreases in rates of protein synthesis.

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References

  1. Agrawal, H. C., and A. N. Davison: In W. Himwich (ed.): Biochemistry of the developing brain. Vol. 1, p. 143 New York: Marcel Dekker (1973).

  2. Brubaker, P. E., R. Klein, S. P. Herman, G. W. Lucier, L. T. Alexander, and M. D. Long: DNA, RNA, and protein synthesis in brain, liver, and kidneys of asymptomatic methylmercury treated rats. Exp. and Mol. Path.18, 263 (1973).

  3. Cavanagh, J. B., and K. C. K. Chen: Amino acid incorporation in protein during the “silent phase” before organo-mercury and p-bromophenylacetylurea neuropathy in the rat. Acta Neuropath.19, 216 (1971).

  4. Dunlop, D. S., W. van Elden, and A. Lajtha: Developmental effects on protein synthesis rates in regions of the CNSin vivo andin vitro. J. Neurochem.29, 939 (1977).

  5. Eayrs, J. T.: Developmental relationships between brain and thyroid. In R. P. Michael (ed.): Endocrinology and human behavior, p. 239. London: Oxford University Press (1968).

  6. Fehling, C., M. Abdulla, A. Brun, M. Dictor, A. Schutz, and S. Skerfving: Methylmercury poisoning in the rat: A combined neurological, chemical, and histopathological study. Toxicol. and Appl. Pharmacol.33, 27 (1975).

  7. Gaitonde, M. K., and R. E. Martenson: Metabolism of highly basic proteins of rat brain during postnatal development. J. Neurochem.17, 551 (1970).

  8. Himwich, H. E.: Early studies on developing brain, In W. Himwich (ed.): Biochemistry of the developing brain. Vol. 1, p. 1. New York: Marcel Dekker (1973).

  9. Kovács, S., W. A. Cocks, and R. Balázs: Incorporation of 2-14C thymidine into deoxyribonucleic acid of rat brain during postnatal development: Effect of thyroid hormone. Biochem. J.114, 60 (1969).

  10. Lehotsky, K., and I. Meszaros: Alteration of electroencephalogram and evoked potential in rats induced by organic mercury. Acta Pharmacol, et Toxicol.35, 180 (1974).

  11. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall: Protein measurement with the Folin reagent. J. Biol. Chem.193, 265 (1951).

  12. Miller, S. A.: Protein metabolism during growth and development, In H. N. Munro (ed.): Mamalian protein metabolism, Vol. 3. New York: Academic Press (1969).

  13. Oja, S. S.: Studies on protein metabolism in developing rat brain. Ann. Acad. Sci. Fenn.131, 7 (1967).

  14. Sobotka, T. J., M. P. Cook, and R. E. Brodie: Effects of perinatal exposure to methylmercury on functional brain development and neurochemistry. Biol. Psy.8, 307 (1974).

  15. Spyker, J. M.: Assessing the impact of low level chemicals on development: Behavioral and latent effects. Fed. Proc.34, 1835 (1975).

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Joiner, F.E., Hupp, E.W. Developmental and methylmercury effects on brain protein synthesis. Arch. Environ. Contam. Toxicol. 8, 465–470 (1979). https://doi.org/10.1007/BF01056353

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Keywords

  • Chloride
  • Waste Water
  • Protein Synthesis
  • Water Management
  • Water Pollution