Factors affecting the removal of metals during activated sludge wastewater treatment II. The role of mixed liquor biomass

  • P. S. Lawson
  • R. M. Sterritt
  • J. N. Lester
Article
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Abstract

Samples of mixed liquor from a laboratory-scale activated sludge simulation, operated at a range of sludge ages from 3 to 12 days, were treated with formaldehyde in order to obtain a metabolically inactive biomass. The metal removal behavior of this biomass was compared with that of untreated biomass. Only Cu and Ni were found to exhibit a high degree of removal in the presence of active biomass. Manganese, Cd, Co, and Tl demonstrated removals little affected by the activity of the biomass, and at longer sludge ages more metal was taken up by formaldehyde-treated than by untreated cells.

Dispersed mixed liquor in the form of a bulking sludge was found to have a greater affinity for most metals than a well-settled, compact mixed liquor. Consequently, it is proposed that the important factors in metal removal by the mixed liquor solids were related to their behavior as particulates, the physical characteristics of the particle being more important than their viability.

Keywords

Biomass Formaldehyde Waste Water Sludge Manganese 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Barrow NJ (1978) The description of phosphate adsorption curves. J Soil Sci 29:447Google Scholar
  2. Bitton G, Freihofer U (1978) Influence of extracellular polysaccharides on the toxicity of copper and cadmium towardsKlebsiella aerogenes. Microb Ecol 4:119Google Scholar
  3. Brown MJ, Lester JN (1982) Role of bacterial extracellular polymers in metal uptake in pure bacterial culture and activated sludge. II. Effects of mean cell residence time. Water Res 16:1549Google Scholar
  4. Bucheder F, Broda E (1974) Energy dependent zinc-transport byEscherichia coli. Eur J Biochem 45:555PubMedGoogle Scholar
  5. Burton K (1955) A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric determination of DNA. Biochem J 62:315Google Scholar
  6. Cheng MH, Patterson JW, Minear PA (1975) Heavy metals uptake by activated sludge. J Water Pollut Control Fed 47:362PubMedGoogle Scholar
  7. Friedman BA, Dugan PR (1968) Concentration and accumulation of metallic ions by the bacteriumZoogloea. Develop Ind Microbiol 9:381Google Scholar
  8. Gadd GM, Mowill JL (1983) The relationship between cadmium uptake, potassium release and viability inSaccharomyces cerevisiae. Microbiol Lett 16:45Google Scholar
  9. Griffin RF, Au AK (1977) Lead adsorption by montmorillonite using a competitive Langmuir equation. Soil Sci Soc Am J 41:880Google Scholar
  10. Kelly DP, Norris PR, Brierley CL (1979) Microbiological methods for the extraction and recovery of metals. In Microbial Technology: Current state, future prospects. Soc. for Gen. Microbiol. Symp. No. 29, Ed Bull A. T., D. C. Ellwood and C. Ratledge, University Press: 263 CambridgeGoogle Scholar
  11. Kushner DJ (1971) Influence of solutes and ions on micro-organisms. In Hugo WB (ed) Inhibition and destruction of the microbial cell. p. 259 Academic Press: LondonGoogle Scholar
  12. Lawson PL, Sterritt RM, Lester JN (1984) Factors affecting the removal of metals during activated sludge wastewater treatment I. The role of soluble ligands. Arch Environ Contam Toxicol 13:383–390Google Scholar
  13. Lester JN, Perry R, Dadd AH. (1979) The influence of heavy metals on a mixed bacterial population of sewage origin in the chemostat. Water Res 13:1055Google Scholar
  14. Lowry OH, Rosebrough NJ, Lewisfarr A, Randall RJ (265) Protein measurement with the phenol reagent. J Biol Chem 193:265Google Scholar
  15. Macaskie LE, Dean ACR (1982) Cadmium accumulation by microorganisms. Environ Technol Lett 3:49Google Scholar
  16. McDermott GN, Post MA, Jackson BN, Ettinger MB (1965) Nickel in relation to the activated sludge and anaerobic digestion processes. J Water Pollut Control Fed 37:163Google Scholar
  17. Mitra RS, Grey RH, Chin B, Bernstein IA (1975) Molecular mechanisms of accomodation inEscherichia coli to toxic levels of cadmium. J Bact 121:1180PubMedGoogle Scholar
  18. Mustermann JL, Morand J (1977) Formaldehyde as a preservative of activated sludge. J Water Pollut Control Fed 49:45Google Scholar
  19. Nelson DL, Kennedy EP (1971) Magnesium transport inEscherichia coli inhibition by cobaltous ion. J Biol Chem 246:3042PubMedGoogle Scholar
  20. Nelson PO, Chung AK, Hudson MC (1981) Factors affecting the fate of heavy metals in the activated sludge process. J Water Pollut Control Fed 53:1323Google Scholar
  21. Norris PR, Kelly DP (1977) Accumulation of cadmium and cobalt bySaccharomyces cerevisiae. J Gen Microbiol 99:317Google Scholar
  22. Paton WH, Budd K (1972) Energy dependent zinc-transport byEscherichia coli. Eur J Biochem 45:555Google Scholar
  23. Perrin DD, Dempsey B (1974) Metal ion buffers. Chap. 7. Chapman and Hall: LondonGoogle Scholar
  24. Pickett AW, Dean ACR (1976) Cadmium and zinc sensitivity and tolerance inKlebsiella (Aerobacter) aerogenes. Microbios 15:79PubMedGoogle Scholar
  25. Pike EB, Carrington EG (1972) Recent developments in the study of bacteria from activated sludge. Water Pollut Control 71:583Google Scholar
  26. Silver S, Budd K, Leahy KM, Shaw WV, Hammond D, Norvick RP, Willsky GR, Malamy MH, Rosenberg H (1981) Inducible plasmid determined resistance to arsenate, arsenite and antimony (III) inEscherichia coli andStaphylococcus aureus. J Bacteriol 146:983PubMedGoogle Scholar
  27. Silver S, Johnseine P, King K (1970) Manganese active transport inEscherichia coli. J Bacteriol 104:1299Google Scholar
  28. Sposito G (1979) Derivation of the Langmuir equation for ion exchange reactions in soils. Soil Sci Soc Am J 43:197Google Scholar
  29. Sterritt RM, Brown MJ, Lester JN (1981) Metal removal by adsorption and precipitation in the activated sludge process. Environ Pollut (Series A) 24:313Google Scholar
  30. Stoveland S, Lester JN (1980) A study of the factors which influence metal removal in the activated sludge process. Sci Total Environ 16:37Google Scholar
  31. Tynecka Z, Zajac J, Gos Z (1981) Energy dependent efflux of cadmium coded by a plasmid resistance determinant inStaphylococcus aureus. J Bacteriol 147:313PubMedGoogle Scholar
  32. Veith JA, Sposito G (1977) On the use of the Langmuir equation in the interpretation of “adsorption” phenomena. Soil Sci Soc Am J 41:697Google Scholar
  33. Webb M (1970) Interrelationships between the utilization of magnesium and the uptake of other bivalent cations by bacteria. Biochim Biophys Acta 222:428PubMedGoogle Scholar
  34. Weiss AA, Silver S, Kinscherf TG (1978) Cation transport alteration associated with plasmid-determined resistance to cadmium inStaphylococcus aureus. Antimicrob Agents Chemother 14:856PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1984

Authors and Affiliations

  • P. S. Lawson
    • 1
  • R. M. Sterritt
    • 1
  • J. N. Lester
    • 1
  1. 1.Public Health Engineering Laboratory, Department of Civil EngineeringImperial College of Science and TechnologyLondonUK

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