Changes in brain dopamine levels, oocyte growth and spermatogenesis in rainbow trout,Oncorhynchus mykiss, following sublethal cyanide exposure

  • Attila Szabo
  • Sylvia M. Ruby
  • Frank Rogan
  • Zalman Amit
Article
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Abstract

Whole brain dopamine (DA) and norepinephrine (NE) levels were measured in sexually maturing (2 years+) male and female rainbow trout,Oncorhynchus mykiss, following ≍posure to 0.01 mg/L hydrogen cyanide (HCN). Following a 12 day exposure period in July and August 1988, whole brain DA levels of HCN exposed fish were significantly higher (p < 0.05), relative to control fish, as measured by high performance liquid chromatography (HPLC). Brain NE levels were unaffected by HCN exposure. Whole brain DA and NE levels showed a strong correlation in control fish (r=+0.81), but not in HCN exposed fish (r=+0.28), likely due to altered DA levels in the latter group. No significant differences were found in brain DA and NE levels between males and females. Mean diameters of oocyte from ovaries of the vitellogenic females were significantly (p < 0.01) reduced from 226 to 183 μm in control and HCN exposed fish respectively. Testes from males revealed significantly (p < 0.001) higher numbers of spermatogonial cysts in HCN exposed fish. Evidence is given that chronic exposure to sublethal levels of HCN significantly alters brain DA levels in both sexes of rainbow trout, reduces growth in vitellogenic oocytes of the ovary in females and interferes with the passage of spermatogonia to the spermatocyte stage in sexually maturing males. Collectively, these results suggest that sublethal HCN affects the reproductive mechanisms via the hypophyseal-gonad axis in sexually maturing rainbow trout.

Keywords

High Performance Liquid Chromatography Rainbow Trout Hydrogen Cyanide Oocyte Growth Brain Dopamine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag New York Inc. 1991

Authors and Affiliations

  • Attila Szabo
    • 1
  • Sylvia M. Ruby
    • 1
  • Frank Rogan
    • 2
  • Zalman Amit
    • 2
  1. 1.Department of BiologyConcordia UniversityMontrealCanada
  2. 2.Center for Studies in Behavioral NeurobiologyConcordia UniversityMontrealCanada

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