Manganese-induced biochemical changes in growing versus adult rats

  • Girja S. Shukla
  • M. P. Dubey
  • Satya V. Chandra


Manganese chloride was administered daily intraperitoneally to growing and adult rats for a period of 30 days to compare certain biochemical parameters in both groups of animals. Marked alterations in the levels of dopamine and norepinephrine in the brain was observed in manganese-treated growing rats. Furthermore, the contents of tyrosine, tryptophan in blood, liver, and brain have been significantly altered in these animals. Identical manganese administration to adult rats had no effect on the contents of tyrosine in liver and brain, nor tryptophan, and dopamine in the brain. Some of the parameters which were altered in the adult rats were less in magnitude compared to the growing animals. The experiments indicated higher susceptibility of the growing rats to the toxic effects of this metal.


Chloride Waste Water Dopamine Tyrosine Manganese 
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  1. Badawy, A. A. B., and M. J. H. Smith: Changes in liver tryptophan and tryptophan pyrrolase activity after administration of salicylate and tryptophan to the rats. Biochem. Pharmacol.21, 97 (1972).Google Scholar
  2. Bagchi, S. P., and Zaryeki Edwin.In vivo formation of tyrosine from phenylalanine in brain. Life Science9, 111 (1970).Google Scholar
  3. Bonilla, E., and M. Diez-Ewald: Effect of L. DOPA on brain concentration of dopamine and homovanillic acid in rats after chronic manganese chloride administration. J. Neurochem.22, 297 (1974).Google Scholar
  4. Chandra, S. V., and G. S. Shukla: Manganese encephalopathy in growing rats. Environ. Res.15, 28 (1978).Google Scholar
  5. Cotzias, G. C., P.S. Papavasiliou, I. Mena, L. C. Tang, and S. T. Miller: Manganese and catecholamines. In F. McDowell and A. Barbeau (ed.): Advances in neurology. Vol. 5, p. 235. New York: Raven Press (1974).Google Scholar
  6. Denckla, W. D., and H. K. Dewey: The determination of tryptophan in plasma, liver and brain. J. Lab. Clin. Med.69, 160 (1967).Google Scholar
  7. Donaldson, J., T. S. T. Pierre, J. L. Minnich, and A. Barbeau: Determination of Na+, K+, Mg2+, Cu2+, Zn++, and Mn2+ in rat brain regions. Canad. J. Biochem.51, 87 (1973).Google Scholar
  8. Granner, D. K., and G. M. Tomkins: Tyrosine aminotransferase (Rat liver). In S. P. Colowick and N. O. Kaplan (ed.): Methods in enzymology Vol. XVII A, p. 633. New York-London: Academic Press (1970).Google Scholar
  9. Haubrich, R. D., and J. S. Denzer: Simultaneous extraction and fluorometric measurement of brain serotonin, catecholamine, 5-hydroxyindoleacetic acid, and homovanillic acid. Anal. Biochem.55, 306 (1973).Google Scholar
  10. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall: Protein measurement with Folin phenol reagent. J. Biol. Chem.193, 265 (1951).Google Scholar
  11. Medical and biological effects of environmental pollutants. “Manganese”. Committee on biologic effects of atmospheric pollutants. National Academy of Sciences: Washington, DC (1973).Google Scholar
  12. Mustafa, S. J., and S. V. Chandra: Levels of 5-HT, dopamine, and norepinephrine in whole brain of rabbits in chronic manganese toxicity. J. Neurochem.18, 931 (1971).Google Scholar
  13. Neff, N. H., R. E. Barrett, and E. Costa: Selective depletion of caudate nucleus dopamine and serotonin during chronic manganese dioxide administration to squirrel monkeys, Experientia.25, 1140 (1969).Google Scholar
  14. Sadavongvivad, C.: Pharmacological significance of biogenic amines in the lungs: 5-Hydroxytryptamine. Brit. J. Pharmacol.38, 353 (1970).Google Scholar
  15. Seth, P. K., R. Hussain, M. Mushtaq, and S. V. Chandra: Effect of manganese on neonatal rat: Manganese concentration and enzymatic alterations in brain. Acta Pharmacol, et. Toxicol.40, 353 (1977).Google Scholar
  16. Waalkes, T. P., and S. Udenfriend: A fluorometric method for the estimation of tyrosine in plasma and tissues. J. Lab. Clin. Med.50, 733 (1957).Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1980

Authors and Affiliations

  • Girja S. Shukla
    • 2
  • M. P. Dubey
    • 1
  • Satya V. Chandra
    • 2
  1. 1.Central Drug Research InstituteLucknowIndia
  2. 2.Industrial Toxicology Research CentreLucknowIndia

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