Chlorinated hydrocarbon levels in human serum: Effects of fasting and feeding
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Twenty healthy adult humans had serum samples drawn on four occasions within a 24-hr period: after a 12 hr overnight fast, 4–5 hr after a high fat breakfast, at midafternoon, and the next morning after another 12 hr fast. Nonfasting samples had 22% to 29% higher mean concentrations (p < 0.05) than did fasting samples for polychlorinated biphenyls (PCBs, 4.81 vs 3.74 ng/g serum wt), hexachlorobenzene (HCB, 0.163 vs 0.134 ng/g serum wt), andp,p′-dichlorodiphenyldichloroethylene (p,p′-DDE, 6.74 vs 5.37 ng/g serum wt) measured by electron capture gas liquid chromatography. Total serum lipids were estimated from measurements of total cholesterol, free cholesterol, triglycerides, and phospholipids and were 20% higher in nonfasting samples than in fasting samples (7.05 g/L vs 5.86 g/L). When PCBs, HCB, andp,p′-DDE concentrations were corrected by total serum lipids, results from fasting and nonfasting samples were not statistically different. Because of the differences in these chlorinated hydrocarbon concentrations observed with different sample collection regimens, meaningful comparison of analytical results requires standardizing collection procedures or correcting by total serum lipid levels.
KeywordsPCBs Free Cholesterol Serum Lipid Level Hexachlorobenzene Hydrocarbon Concentration
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- Brown JF, Lawton RW (1984) Polychlorinated biphenyl (PCB) partitioning between adipose tissue and serum. Bull Environ Contain Toxicol 33:277–280Google Scholar
- Cooper GR, Duncan PH, Hazelhurst JS, Miller DT, Bayse DD (1982) Cholesterol. Enzymic Method. In:Faulkner WR, Meites S (eds) Selected Methods for the Small Clinical Chemistry Laboratory, Vol 9. American Association for Clinical Chemistry, Washington, DC, pp 165–174Google Scholar
- Matthews HB (1980) Disposition of persistent halogenated hydrocarbons in higher animals. In:Khan MAQ, Stanton RH (eds) Toxicology of halogenated hydrocarbons:Health and ecological effects. Pergamon, New York, p 289–297Google Scholar
- Matthews HB, Anderson MW (1975) Effect of chlorination on the distribution and excretion of polychlorinated biphenyls. Drag Metab Dispos 3:371–380Google Scholar
- Matthews HB, Dedrick RL (1984) Pharmacokinetics of PCBs. Ann Rev Pharmacol Toxicol 24:85–103Google Scholar
- Matthews HB, Surles JR, Carver JG, Anderson MW (1984) Halogenated biphenyl transport by blood components. Fund Appl Toxicol 4:420–428Google Scholar
- National Heart, Lung, and Blood Institute (1980) The Lipid Research Clinics Population Studies Data Book, Vol 1, The Prevalence Study. NIH Publication 80-1527Google Scholar
- Patterson DG, Needham LL, Pirkle JL, Roberts DW, Bagby J, Garrett WA, Andrews JS, Falk H, Bernert JT, Sampson EJ, Houk VN (1988). Correlation between serum and adipose tissue levels of 2,3,7,8-tetrachlorodibenzo-p-dioxin in 50 persons from Missouri. Arch Environ Contam Toxicol 17:139–143PubMedGoogle Scholar
- Safe S (1984) Polychlorinated biphenyls (PCBs) and polybrominated biphenyls (PBBs):Biochemistry, toxicology, and mechanism of action. CRC Crit Rev Toxicol 13:319–365Google Scholar
- SAS Institute, Inc (1985) SAS User's Guide:Statistics, Version 5. SAS Institute, Inc., Cary, NCGoogle Scholar
- Sokal RR, Rohlf FJ (1969) Biometry. WH Freeman, San FranciscoGoogle Scholar