Glycoconjugate Journal

, Volume 13, Issue 1, pp 27–31

Glycosylation ofα1 glycoprotein in septic shock: Changes in degree of branching and in expression of sialyl Lewisx groups

  • Els C. M. Brinkman-Van Der Linden
  • Esther C. R. Van Ommen
  • Willem Van Dijk
Glycosylation and Disease Papers

DOI: 10.1007/BF01049676

Cite this article as:
Brinkman-Van Der Linden, E.C.M., Van Ommen, E.C.R. & Van Dijk, W. Glycoconjugate J (1996) 13: 27. doi:10.1007/BF01049676

Abstract

The occurrence of differences in acute-phase response, with respect to concentration and glycosylation of α1-acid glycoprotein (AGP) was studied in the sera of patients surviving or not from septic shock. Crossed affino-immunoelectrophoresis was used with concanavalin A andAleuria aurantia lectin for the detection of the degree of branching and fucosylation, respectively, and the monoclonal CSLEX-1 for the detection of sialyl Lewisx (SLeX) groups on AGP. Septic shock apparently induced an acute-phase response as indicated by the increased serum levels and changed glycosylation of AGP. In the survivor group a transient increase in diantennary glycan content was accompanied by a gradually increasing fucosylation and SLeX expression, comparable to those observed in the early phase of an acute-inflammatory response. Remarkably, in the non-survivor group a modest increase in diantennary glycan content was accompanied by a strong elevation of the fucosylation of AGP and the expression of SLeX groups on AGP, typical for the late phase of an acute-phase response. Our results suggest that these changes in glycosylation of AGP can have a prognostic value for the outcome of septic shock.

Keywords

α1-acid glycoprotein orosomucoid septic shock sepsis sialyl Lewisx fucosylation concanavalin A Aleuria aurantia lectin 

Abbreviations

AAL

Aleuria aurantia lectin

AGP

α1-acid glycoprotein

CAIE

crossed affinoimmunoelectrophoresis

Con A

Concanavalin A

HSPC

human serum protein calibrator

IL-1

interleukin 1

IL-6

interleukin 6

LIF

leukaemia inhibitory factor

LPS

lipopolysaccharide

SLeX

sialyl Lewisx

TNF

tumour necrosis factor

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Els C. M. Brinkman-Van Der Linden
    • 1
  • Esther C. R. Van Ommen
    • 1
  • Willem Van Dijk
    • 1
  1. 1.Department of Medical Chemistry, Faculty of MedicineVrije UniversiteitAmsterdamThe Netherlands

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