Journal of Insect Behavior

, Volume 4, Issue 5, pp 545–556

Dietary mixing in grasshoppers: Changes in acceptability of different plant secondary compounds associated with low levels of dietary protein (Orthoptera: Acrididae)

  • E. A. Bernays
  • D. Raubenheimer
Article

Abstract

Schistocerca americanasixth-instar nymphs were examined for a change in diet acceptance, in which insects experiencing an unfavorable diet subsequently become predisposed to eat relatively less of that diet and more of diets with a novel flavor than they would had they previously fed on a more adequate diet. Insects were pretreated for 4 h on either low-protein (2 % wet wt) or higherprotein (4%) artificial diets flavored with a plant secondary compound (tomatine or rutin). They were then offered, in choice or no-choice tests, the lowprotein diet with the familiar or a novel (tomatine, rutin, or NHT) flavor. When tomatine was the familiar and rutin the novel flavor in a no-choice test, the insects previously fed low-protein diets took relatively long meals on the novel and relatively short meals on the familiar diets compared with the insects that had previously eaten higher-protein diets. A similar, but in this case considerably less pronounced and statistically nonsignificant, pattern existed in the reciprocal design experiment in which rutin was the familiar and tomatine the novel flavor. Similarly, insects fed low-protein diets flavored with rutin subsequently showed an increased relative preference for the novel flavor (NHT) in a choice test, compared with the high protein-pretreated insects. It is concluded that insects fed protein-deficient diets may subsequently show a preference for novel foods through different mechanisms, the importance of which may differ in different circumstances.

Key words

Schistocerca grasshopper learning aversion novelty polyphagy dietary mixing 

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References

  1. Abisgold, J. D., and Simpson, S. J. (1987). The physiology of compensation by locusts for changes in dietary protein.J. Exp. Biol. 129: 329–346.Google Scholar
  2. Abisgold, J. D., and Simpson, S. J. (1988). The effect of dietary protein levels and haemolymph composition on the sensitivity of the maxillary palp chemoreceptors of locusts.J. Exp. Biol. 135: 215–229.Google Scholar
  3. Bernays, E. A., and Lee, J. C. (1988). Food aversion learning in the polyphagous grasshopper,Schistocerca americana.Physiol. Entomol. 13: 131–137.Google Scholar
  4. Bernays, E. A., Howard, J. J., Champagne, D., and Estesen, B. (1991). Rutin: A phagostimulant for the polyphagous acrididSchistocerca americana. Entomol. Exp. Appl. (in press).Google Scholar
  5. Champagne, D., and Bernays, E. A. (1991). Phytosterol unsuitability as a factor mediating food aversion learning in the grasshopperSchistocerca americana. Physiol. Entomol. (in press).Google Scholar
  6. Cohen, R. W., Friedman, S., and Waldbauer, G. P. (1987). Physiological control of nutrient selfselection inHeliothis zea larvae: the role of serotonin.J. Insect Physiol. 34: 935–940.Google Scholar
  7. Cook, A. G. (1976). A critical review of the methodology and interpretation of experiments designed to assay the phagostimulatory activity of chemicals to phytophagous insects.Symp. Biol. Hung. 16: 47–54.Google Scholar
  8. Geissler, T. G., and Rollo, C. D. (1988). The influence of nutritional history on the response to novel food by the cockroachPeriplaneta americana.Anim. Behav. 35: 1905–1907.Google Scholar
  9. Heinrichs, S. C., Deutch, J. A., and Moore, B. O. (1990). Olfactory self-selection of proteincontaining foods.Physiol. Behav. 47: 409–413.PubMedGoogle Scholar
  10. Hunter-Jones, P. (1961).Rearing and Breeding Locusts in the Laboratory, Anti-Locust Research Centre, London.Google Scholar
  11. Lee, J. C., and Bernays, E. A. (1988). Declining acceptability of a food plant for the polyphagous grasshopperSchistocerca americana: The role of food aversion learning.Physiol. Entomol. 13: 291–301.Google Scholar
  12. Lee, J. C., and Bernays, E. A. (1990). Food tastes and toxic effects: Associative learning by the polyphagous grasshopperSchistocerca americana (Drury) (Orthoptera: Acrididae)Anim. Behav. 39: 163–173.Google Scholar
  13. McFarland, D. (1985).Animal Behavior, Benjamin Cummings, Menlo Park, Calif.Google Scholar
  14. Raubenheimer, D., and Simpson, S. J. (1990). The effects of simultaneous variation in protein, digestible carbohydrate and tannic acid on the feeding behaviour of larvalLocusta migratoria (L.) andSchistocerca gregaria (Forskal). I. Short-term studies.Physiol. Entomol. 15: 219–223.Google Scholar
  15. Rozin, P. (1976). The selection of foods by rats, humans and other animals. In Rosenblatt, J. S., Hinde, R. A., Shaw, E., and Bear, C. (eds.),Advances in the Study of Behavior, Vol. 6, Academic Press, New York, pp. 21–76.Google Scholar
  16. Simpson, S. J., and Abisgold, J. D. (1985). Compensation by locusts for changes in dietary nutrients: Behavioural mechanisms.Physiol. Entomol. 10: 443–452.Google Scholar
  17. Simpson, S. J., and Simpson, C. L. (1990). The mechanisms of nutritional compensation by phytophagous insects. In Bernays, E. A. (ed.),Insect-Plant Interactions, Vol. II, CRC Press, Boca Raton, Fla., pp. 111–160.Google Scholar
  18. Simpson, S. J., and White, P. (1990). Associative learning and locust feeding: Evidence for a “learned hunger” for protein.Anim. Behav. 40: 506–513.Google Scholar
  19. Simpson, S. J., Simmonds, M. S. J., Blaney, W. M., and Jones, J. P. (1990). Compensatory dietary selection occurs in larvalLocusta migratoria but notSpodoptera littoralis after a single deficient meal duringad libidum feeding.Physiol. Entomol. 15: 235–242.Google Scholar
  20. Waldbauer, G. P., and Friedman, S. (1988). Dietary self-selection in insects. In Sehnal, F., Zabza, A., and Denlinger, D. L. (eds.),Frontiers in Physiological Insect Ecology, Wroclaw Technical University Press, Wroclaw, pp. 403–442.Google Scholar

Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • E. A. Bernays
    • 1
  • D. Raubenheimer
    • 1
  1. 1.Department of EntomologyUniversity of ArizonaTucson

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