Biotechnology Letters

, Volume 11, Issue 10, pp 699–704 | Cite as

Production of cloned carboxypeptidase G2 by Escherichia coli: Genetic and environmental considerations

  • M. J. Lancaster
  • R. J. Sharp
  • J. R. Court
  • I. D. McEntee
  • R. G. Melton
  • R. Sherwood
Article

Summary

The optimum production of cloned carboxypeptidase G2 from plasmid pNM21 byEscherichiacoli was found to be strongly strain- and temperature-dependent. The superior host was strain RV308 and the preferred growth temperature 28°C. Copy number, which decreased during exponential growth of all strains examined, did not relate in these studies to the level of enzyme production: the strain with the highest enzyme yield also having the lowest overall copy number.

Keywords

Enzyme Escherichia Coli Exponential Growth Growth Temperature Enzyme Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • M. J. Lancaster
    • 1
  • R. J. Sharp
    • 1
  • J. R. Court
    • 1
  • I. D. McEntee
    • 1
  • R. G. Melton
    • 1
  • R. Sherwood
    • 1
  1. 1.Division of BiotechnologyPHLS, Centre for Applied Microbiology and ResearchSalisbury

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