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Biotechnology Letters

, Volume 11, Issue 10, pp 695–698 | Cite as

Production of human leukocyte interferon inEscherichiacoli by control of growth rate in fed-batch fermentation

  • Lee Jae-Ho
  • Youn-Hee Choi
  • Shin-Kwon Kang
  • Hyung-Hwan Park
  • Ik-Boo Kwon
Article

Summary

AnE. coli harboring a vector containing double promoters, a signal sequence, and interferon gene was used. By fitting the feed rate of growth-limiting nutrients to the precalculated demand of the microorganism on the basis of a specific growth rate of 0.1 h−1, fed-batch fermentations were performed. A cell density of 26 g/L was achieved after 46 hrs cultivation at 30°C. The culture was induced by IPTG and produced 1x109 IU/L of human leukocyte interferon.

Keywords

Growth Rate Fermentation Interferon Cell Density Feed Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Cornelis, P., Digneffe, C., and Willemot, K. (1982).Mol. Gen. Genet., 186, 507–511.PubMedGoogle Scholar
  2. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982). Molecular Cloning, A Laboratory Manual, New York, Cold Spring Harbor Laboratory.Google Scholar
  3. Marston, F. A. O. (1987). The purification of eukaryotic polypeptides experessed inE. coli. In: DNA cloning, D. M. Glover, ed. vol. 3, pp 59–87, IRL Press.Google Scholar
  4. Masui, Y., Coleman, J., and Inouye, M. (1983). Multipurpose expression cloning vehicles inE. coli. In: Experimental manipulation of gene expression, Inouye, M., ed. pp 15–31, Academic Press.Google Scholar
  5. Meyer, H.-P., and Fiechter, A. (1985).Applied Environ. Microbiol. 50, 503–507.Google Scholar
  6. Nelson, M. J. (1944).J. Biol. Chem., 124, 1375.Google Scholar
  7. Wang, H. Y., Cooney, C. L., and Daniel, I. (1979).Biotech. Bioeng., 21, 975–995.Google Scholar
  8. Zabriskie, D. W., and Arcuri, E. J. (1986).Enzyme Microb. Technol., 8, 706–716.Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Lee Jae-Ho
    • 1
  • Youn-Hee Choi
    • 1
  • Shin-Kwon Kang
    • 1
  • Hyung-Hwan Park
    • 1
  • Ik-Boo Kwon
    • 1
  1. 1.Biotechnology SectionLotte Group R&D CenterSeoulKorea

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