Biotechnology Letters

, Volume 9, Issue 2, pp 89–94 | Cite as

Physiological constraints in increasing biomass concentration ofEscherichiacoli B in fed-batch culture

  • Jae Gu Pan
  • Joon Shick Rhee
  • Jean M. Lebeault


Fed-batch culture ofE.coli B was carried out to obtain high concentration of biomass. After requirement of oxygen was met by sparging the pure oxygen, physiological constraints were delineated. High partial pressure of CO2 caused the decrease of the maximum specific growth rate, whereas fermentative byproducts caused the decrease of biomass yield as well as the maximum specific growth rate.


Oxygen Biomass Growth Rate Organic Chemistry Partial Pressure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anderson, K.B., and von Meyenburg K. (1980).J. Bacteriol. 144, 144.Google Scholar
  2. Atkinson, B., and Mavituna, F. (1983). in “Biochemical Engineering &Biotechnology Handbook”, pp. 660, The Nature Press, London.Google Scholar
  3. Carlsson, J. (1973).Appl. Microbiol. 25, 287.Google Scholar
  4. Fiechter, A. (1984).Adv. Biochem. Eng./Biotechnol. 30, 8.Google Scholar
  5. Guirard, B.M., and Snell, E.E. (1981). in “Manuals of Methods for General Bacteriology”, pp. 79, ASM.Google Scholar
  6. Landwall, P., and Holm, T. (1977).J. Gen. Bacteriol. 103, 345.Google Scholar
  7. Mori, H., Yano, T., Kobayashi, T., and Shimizu, S. (1979).J. Chem. Eng. Japan. 12, 313.Google Scholar
  8. Mori, H., Yamane, T., Kobayashi, T., and Shimizu, S. (1983)J. Ferment. Technol. 61, 391.Google Scholar
  9. Pan, J.G. (1985). “High Cell Density Culture ofEscherichia coli B andRhodotorula glutinis: Kinetic and Energetic Approaches”, Ph.D. Thesis, KAIST, Seoul, Korea.Google Scholar
  10. Teixeira de Mattos, M.J., Neijssel, O.M., and Tempest, D.W. (1982). in “Overproduction of Microbial Products,”, Eds. V. K. Krumphanzl, B. Sikyta, and Z. Vanek., pp. 581 AP, London.Google Scholar
  11. Yano, T., Kobayashi, T., and Shimizu, S. (1981)J. Ferment. Technol. 59, 295.Google Scholar

Copyright information

© Kluwer Academic Publishers 1987

Authors and Affiliations

  • Jae Gu Pan
    • 1
  • Joon Shick Rhee
    • 1
  • Jean M. Lebeault
    • 2
  1. 1.Genetic Engineering Center and Department of Biological Science and EngineeringKorea Advanced Institute of Science and TechnologySeoulKorea
  2. 2.Division de Procédés BiotéchnologiquesUniversité de Téchnologie de CompièngneCompièngneFrance

Personalised recommendations