Physiological constraints in increasing biomass concentration ofEscherichiacoli B in fed-batch culture
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Fed-batch culture ofE.coli B was carried out to obtain high concentration of biomass. After requirement of oxygen was met by sparging the pure oxygen, physiological constraints were delineated. High partial pressure of CO2 caused the decrease of the maximum specific growth rate, whereas fermentative byproducts caused the decrease of biomass yield as well as the maximum specific growth rate.
KeywordsOxygen Biomass Growth Rate Organic Chemistry Partial Pressure
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