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Biotechnology Letters

, Volume 9, Issue 2, pp 89–94 | Cite as

Physiological constraints in increasing biomass concentration ofEscherichiacoli B in fed-batch culture

  • Jae Gu Pan
  • Joon Shick Rhee
  • Jean M. Lebeault
Article

Summary

Fed-batch culture ofE.coli B was carried out to obtain high concentration of biomass. After requirement of oxygen was met by sparging the pure oxygen, physiological constraints were delineated. High partial pressure of CO2 caused the decrease of the maximum specific growth rate, whereas fermentative byproducts caused the decrease of biomass yield as well as the maximum specific growth rate.

Keywords

Oxygen Biomass Growth Rate Organic Chemistry Partial Pressure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1987

Authors and Affiliations

  • Jae Gu Pan
    • 1
  • Joon Shick Rhee
    • 1
  • Jean M. Lebeault
    • 2
  1. 1.Genetic Engineering Center and Department of Biological Science and EngineeringKorea Advanced Institute of Science and TechnologySeoulKorea
  2. 2.Division de Procédés BiotéchnologiquesUniversité de Téchnologie de CompièngneCompièngneFrance

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