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Use of macroreticular resins for the adsorption of urokinase from human urine

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Summary

22 commercial macroreticular resins were screened for their suitability to adsorb the enzyme urokinase directly from human urine at the outlet of a urinal. Both batch and fixed bed techniques were used for the screening, which identified a phenolic resin (Duolite S-762) as having the best capacity and adsorption efficiency. A capacity in excess of 1800 IU/ml. resin is attainable, and elution with 50% ethanolamine followed by immediate neutralisation with HCl gives satisfactory recovery of activity.

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Authors and Affiliations

  1. Department of Chemical Engineering, Pembroke St., CB2 3RA, Cambridge, England

    B. J. Horstmann, A. P. Cameron, C. N. Kenney & H. A. Chase

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  1. B. J. Horstmann
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  2. A. P. Cameron
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  3. C. N. Kenney
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  4. H. A. Chase
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Horstmann, B.J., Cameron, A.P., Kenney, C.N. et al. Use of macroreticular resins for the adsorption of urokinase from human urine. Biotechnol Lett 7, 307–312 (1985). https://doi.org/10.1007/BF01030276

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  • DOI: https://doi.org/10.1007/BF01030276

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