Biotechnology Letters

, Volume 14, Issue 9, pp 811–816 | Cite as

Production of poly-β-hydroxybutyrate by fed-batch culture of recombinantEscherichia coli

  • Beom Soo Kim
  • Sang Yup Lee
  • Ho Nam Chang

Summary

A recombinantEscherichia coli strain harboring the PHB biosynthesis genes fromAlcaligenes eutrophus was used to produce poly-β-hydroxybutyrate (PHB) by pH-stat fedbatch culture. Initial glucose concentration for optimal growth was found to be 20g/L from a series of flask cultures. A final PHB concentration of 88.8 g/L could be obtained after 42 hrs of cultivation.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Braunegg, G., Sonnleitner, B., and Lafferty, R.M. (1978).Eur. J. Appl. Microbiol. Biotechnol. 6, 29–37.Google Scholar
  2. Byrom, D. (1987).Trends Biotechnol. 5, 246–250.Google Scholar
  3. Dawes, E., and Senior, P. (1973).Adv. Microb. Physiol. 10, 135–266.Google Scholar
  4. Harrison, D., and Pirt, S. (1967).J. Gen. Micorobiol. 46, 193–211.Google Scholar
  5. Li, X., Robbins, J., and Taylor, K. (1990).J. Ind. Microbiol. 5, 85–94.Google Scholar
  6. Peoples, O.P., and Sinskey, A.J. (1989).J. Biol. Chem. 264, 15298–15303.Google Scholar
  7. Riesenberg, D., Menzel, K., Schulz, V., Schumann, K., Voith, G., Zuber, G., and Knorre, W. (1990).Appl. Microbiol. Biotechnol. 34, 77–82.Google Scholar
  8. Sambrook, J., Fritsch, E., and Maniatis, T. (1989).Molecular Cloning, A Laboratory Manual. 2nd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.Google Scholar
  9. Schubert, P., Steinbuchel, A., and Schlegel, H. (1988).J. Bacteriol. 170, 5837–5847.Google Scholar
  10. Slater, S., Voige, W., and Dennis, D. (1988).J. Bacteriol. 170, 4431–4436.Google Scholar
  11. Suzuki, T., Yamane, T., and Shimizu, S. (1986).Appl. Microbiol. Biotechnol. 24 370–374.Google Scholar
  12. Suzuki, T., Yamane, T., and Shimizu, S. (1990).J. Ferment. Bioeng. 64, 292–297.Google Scholar
  13. Yamane, T., and Shimizu, S. (1984). Fed-batch Techniques in Microbiol Processes. In:Advances in Biochemical Engineering/Biotechnology, A. Fiechter, ed. vol. 30, pp. 147–194, New York: Springer-Verlag.Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Beom Soo Kim
    • 1
  • Sang Yup Lee
    • 1
  • Ho Nam Chang
    • 1
  1. 1.BioProcess Engineering Research Center and Department of Chemical EngineeringKorea Advanced Institute of Science and TechnologyTaejonKorea

Personalised recommendations