HPLC determination of caffeine and theophylline inPaullinia cupana Kunth (Guarana) and Cola spp. samples

  • Flavio Belliardo
  • Aldo Martelli
  • Maria Grazia Valle
Original Papers


A reversed-phase high-performance liquid-chromatographic method for the determination of caffeine and theophylline in commercial guarana samples (drug obtained from the seeds ofPaulinia cupana Kunth,Sapindaceae of the Amazon Region) and inCola spp. samples is described and discussed.

The methodology developed is simple and rapid with a minimum of samples preparation required. A comparision of five different techniques for the extraction of caffeine and theophylline is discussed. Furthermore the quantitative determination of caffeine and theophylline in five samples of Brasilian guarana, in two samples of dietetic products containing guarana, in two samples ofCola extract and in three ofCola seed powder are reported.


Analytical Chemistry HPLC Sample Preparation Caffeine Theophylline 
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HPLC-Bestimmung von Coffein und Theophyllin inPaullinia cupana- und in Cola spp.-Proben


Die Anwendung einer RP-HPLC für die mengenmässige Bestimmung von Coffein und Theophyllin in Guarana-Proben (Droge aus den Samen desPaullinia cupana-Baumgewächses Kunth, Familie:Sapindaceae) des Amazonas-Gebiet und in Cola spp.-Proben wird beschrieben. Die analytische Methode ist einfach, nicht zeitaufwendig und erfordert nur ein Mindestmaß an analytischen Operationen für die Proben-Zubereitung. Der Vergleich von 5 verschiedenen Extraktionstechniken für Coffein wird ebenfalls erörtert. Außerdem wurde der Gehalt von Coffein und Theophyllin in fünf verschiedenen brasilianischen Guarana-Proben, zwei Proben diätetischer Erzeugnisse mit Guarana-Gehalt, zwei Cola-Extraktund drei Cola-Samen-Proben bestimmt.


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Flavio Belliardo
    • 1
  • Aldo Martelli
    • 1
  • Maria Grazia Valle
    • 1
  1. 1.Istituto di Chimica Farmaceutica e Tossicologica, Laboratorio di R. M. N. e Spettroscopie applicate alla Tossicologia, Facoltà di FarmaciaUniversità di TorinoTorinoItaly

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