Biotechnology Letters

, Volume 13, Issue 5, pp 327–332 | Cite as

Lipase catalysed isomerisation of 1,2-(2,3)-diglyceride into 1,3-diglyceride. The crucial role of water

  • Andreas Heisler
  • Claude Rabiller
  • Laurence Hublin


Enzyme catalyzed isomerisation of 1,2-dipalmitin into the 1,3-isomer is shown to occur with 1,3 regiospecific lipases. The mechanism of this transformation is elucidated and the crucial role of the water still present in the enzyme preparation is shown. Thus, the first step is the hydrolysis of the dipalmitin followed by the isomerisation of the 2-monopalmitin into the 1-monoester. The last is then esterified into the 1,3-dipalmitin.


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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Andreas Heisler
    • 1
  • Claude Rabiller
    • 1
  • Laurence Hublin
    • 1
  1. 1.Laboratoire de RMN et Réactivité Chimique-URA CNRS 472Faculté des Sciences et des TechniquesNantes cédex 03France

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