Journal of Protein Chemistry

, Volume 9, Issue 1, pp 75–82 | Cite as

On the mechanism of activation of protein kinase FA (an activating factor of ATP·Mg-dependent protein phosphatase) in brain myelin

  • Shiaw-Der Yang
  • Jau-Song Yu
  • Chih-Wei Hua
Article

Abstract

Protein kinase FA (an activating factor of ATP·Mg-dependent protein phosphatase) has been characterized to exist in two forms in the purified brain myelin. One form of kinase FA is spontaneously active and trypsin-labile, whereas the other form of kinase FA is inactive and trypsin-resistant, suggesting a different membrane topography with active FA exposed on the outer face of the myelin membrane and inactivu FQ buried within the myelin membrane. When myelin was solubilized in 1% Triton X-100, all kinase FA became active and trypsin-labile. Phospholipid reconstitution studies further indicated that when kinase FA was reconstituted in acidic phospholipids, such as phosphatidylinositol and phosphatidylserine, the enzyme activity was inhibited in a dose-dependent manner, suggesting that kinase FA interacts with acidic phospholipids which inhibit its activity. Furthermore, when myelin was incubated with exogenous phospholipase C, the inactive/trypsin-resistant FA could be converted to the active/trypsin-labile FA in a time- and dose-dependent manner. Taken together, it is concluded that membrane phospholipids play an important role in modulating the activity of kinase FA in the brain myelin. It is suggested that phospholipase C may mediate the activation-sequestration of inactive/trypsin-resistant kinase FA in the brain myelin through the phospholipase C-katalyzed degradation of acidic membrane phospholipids. The activation-sequestration of protein Kinase FA may represent one mode of control modulating the activity of kinase FA in the central nervous system myelin.

Key words

Protein kinase protein phosphatase brain myelin membranes phospholipids 

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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Shiaw-Der Yang
    • 1
    • 2
  • Jau-Song Yu
    • 1
    • 2
  • Chih-Wei Hua
    • 1
    • 2
  1. 1.Institute of Life ScienceNational Tsing Hua UniversityHsinchu
  2. 2.Institute of Molecular Cell BiologyChang Gung Medical CollegeTao-YuanTaiwan, ROC

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