Journal of Protein Chemistry

, Volume 8, Issue 2, pp 159–163 | Cite as

Manual precyclization of fiberglass filters for microsequence analysis of peptides and proteins

  • Billy Touchstone
  • Zi-Wei Gu
  • Chao-Yuh Yang
Article
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  • 18 Downloads

Abstract

A manual procedure for precyclization of fiberglass filters has been described. The quality of the manually precycled filter is as good for microsequence analysis of proteins and peptides as a filter that has been precycled by an automatic system. The manually precycled filter is convenient to use and saves time and money.

Key words

manual precyclization polybrene-coated glass filter gas-phase sequence analysis 
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References

  1. Hewick, R. M., Hunkapiller, M. W., Hood, L. E., and Dreyer, W. J. (1981).J. Biol. Chem. 256, 7990–7997.Google Scholar
  2. Matsudaira, P. (1987).J. Biol. Chem. 262, 10035–10038.Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Billy Touchstone
    • 1
  • Zi-Wei Gu
    • 1
  • Chao-Yuh Yang
    • 1
  1. 1.Baylor College of Medicine and The Methodist HospitalHouston

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