Journal of Protein Chemistry

, Volume 11, Issue 3, pp 321–331

Sequence and structural relationships in the cytokine family

  • Parthasarathy Manavalan
  • Deborah L. Swope
  • Raymond M. Withy
Article

Abstract

The sequences of nine different cytokines, growth hormone, and prolactin have been aligned and their secondary structure predicted. The alignment reveals that each exon has a characteristic sequence pattern shared by all cytokines. The most striking sequence similarity is observed in exon 4, where the residue pair Phe-Leu is conserved in many cytokines. In addition, there are discreet homologous regions between two specific growth factors, including a high degree of homology between granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 3 (IL-3). The secondary structure analysis predicts that exon 3 of all cytokines has an antiparallel helix-turn-helix motif, which is likely to form the central helical segments of a four α-helical bundle-type structure. Based on the secondary structure and the disulfidebonding pattern, the topological connectivity for a number of cytokines has been predicted.

Key words

Cytokines sequence homology secondary structure folding topology 

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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Parthasarathy Manavalan
    • 1
  • Deborah L. Swope
    • 1
  • Raymond M. Withy
    • 1
  1. 1.Genzyme CorporationFramingham

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