Biotechnology Letters

, Volume 9, Issue 10, pp 691–696

Strategies to extend longevity of hybridomas in culture and promote yield of monoclonal antibodies

  • Y. T. Luan
  • R. Mutharasan
  • W. E. Magee
Article

DOI: 10.1007/BF01024599

Cite this article as:
Luan, Y.T., Mutharasan, R. & Magee, W.E. Biotechnol Lett (1987) 9: 691. doi:10.1007/BF01024599

Summary

Cell viability was improved by supplemental feeding of amino acids and vitamins in batch culture of hybridoma cells. Cells could be maintained over a 10 day period following exponential growth at a constant viable cell concentration of 2.1×106 cells/ml. Concentrations of monoclonal antibody (MAb) reached 140 mg/l, a value of nearly four times that found in typical batch culture. Lactate formation appeared to occur only during active exponential growth and not during the stationary phase.

Copyright information

© Science and Technology Letters 1987

Authors and Affiliations

  • Y. T. Luan
    • 1
  • R. Mutharasan
    • 1
  • W. E. Magee
    • 2
  1. 1.Department of Chemical EngineeringDrexel UniversityPhiladelphiaUSA
  2. 2.Department of Bioscience and BiotechnologyDrexel UniversityPhiladelphiaUSA

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