Strategies to extend longevity of hybridomas in culture and promote yield of monoclonal antibodies
- Cite this article as:
- Luan, Y.T., Mutharasan, R. & Magee, W.E. Biotechnol Lett (1987) 9: 691. doi:10.1007/BF01024599
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Cell viability was improved by supplemental feeding of amino acids and vitamins in batch culture of hybridoma cells. Cells could be maintained over a 10 day period following exponential growth at a constant viable cell concentration of 2.1×106 cells/ml. Concentrations of monoclonal antibody (MAb) reached 140 mg/l, a value of nearly four times that found in typical batch culture. Lactate formation appeared to occur only during active exponential growth and not during the stationary phase.